Production of d-lactate from glucose using Klebsiella pneumoniae mutants

doi:10.1186/s12934-017-0822-6

	Production of d-lactate from glucose using Klebsiella pneumoniae mutants
	Feng,Xinjun 1,2; Jiang,Liqun 2; Han,Xiaojuan 1; Liu,Xiutao 1,3; Zhao,Zhiqiang 1,3; Liu,Huizhou 1; Xian,Mo 1; Zhao,Guang 1
刊名	Microbial Cell Factories
	2017-11-21
卷号	16 期号:1
关键词	d-Lactate Acetolactate synthase Acetate kinase Alcohol dehydrogenase Klebsiella pneumoniae
ISSN号	1475-2859
DOI	10.1186/s12934-017-0822-6
英文摘要	AbstractBackgroundd-Lactate is a valued chemical which can be produced by some bacteria including Klebsiella pneumoniae. However, only a few studies have focused on K. pneumoniae for d-lactate production with a significant amount of by-products, which complicated the purification process and decreased the yield of d-lactate.ResultsBased on the redirection of carbon towards by-product formation, the effects of single-gene and multiple-gene deletions in K. pneumoniae on d-lactate production from glucose via acetolactate synthase (budB), acetate kinase (ackA), and alcohol dehydrogenase (adhE) were tested. Klebsiella pneumoniae mutants had different production behaviours. The accumulation of the main by-products was decreased in the mutants. The triple mutant strain had the most powerful ability to produce optically pure d-lactate from glucose, and was tested with xylose and arabinose as carbon sources. Fed-batch fermentation was also carried out under various aeration rates, and the strain accumulated 125.1?g/L d-lactate with a yield of 0.91?g/g glucose at 2.5 vvm.ConclusionsKnocking out by-product synthesis genes had a remarkable influence on the production and yield of d-lactate. This study demonstrated, for the first time, that K. pneumoniae has great potential to convert monosaccharides into d-lactate. The results provide new insights for industrial production of d-lactate by K. pneumoniae.
语种	英语
出版者	BioMed Central
WOS记录号	BMC:10.1186/S12934-017-0822-6
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/16472]
专题	中国科学院广州能源研究所
通讯作者	Liu,Huizhou; Xian,Mo; Zhao,Guang
作者单位	1. 2. 3.
推荐引用方式 GB/T 7714	Feng,Xinjun,Jiang,Liqun,Han,Xiaojuan,et al. Production of d-lactate from glucose using Klebsiella pneumoniae mutants[J]. Microbial Cell Factories,2017,16(1).
APA	Feng,Xinjun.,Jiang,Liqun.,Han,Xiaojuan.,Liu,Xiutao.,Zhao,Zhiqiang.,...&Zhao,Guang.(2017).Production of d-lactate from glucose using Klebsiella pneumoniae mutants.Microbial Cell Factories,16(1).
MLA	Feng,Xinjun,et al."Production of d-lactate from glucose using Klebsiella pneumoniae mutants".Microbial Cell Factories 16.1(2017).