Efficient Separation of Four Antibacterial Diterpenes from the Roots of Salvia Prattii Using Non-Aqueous Hydrophilic Solid-Phase Extraction Followed by Preparative High-Performance Liquid Chromatography

doi:10.3390/molecules23030623

	Efficient Separation of Four Antibacterial Diterpenes from the Roots of Salvia Prattii Using Non-Aqueous Hydrophilic Solid-Phase Extraction Followed by Preparative High-Performance Liquid Chromatography
	Shao, Yun 2,3; Tao, Yanduo 2,3; Wang, Qilan2,3 ; Mei, Lijuan 2,3; Jiao, Lijin 2,3; Wang, Weidong 2,3; Liu, Zenggen2,3 ; Yue, Huilan 2,3; Pei, Jinjin 1,3; Cui, Yulei 2,3
刊名	MOLECULES
	2018-03-01
卷号	23 期号:3
关键词	Salvia Prattii Antibacterial Diterpenes Hydrophilic Solid-phase Extraction Preparative High-performance Liquid Chromatography
ISSN号	1420-3049
DOI	10.3390/molecules23030623
文献子类	Article
英文摘要	An efficient preparative procedure for the separation of four antibacterial diterpenes from a Salvia prattii crude diterpenes-rich sample was developed. Firstly, the XION hydrophilic stationary phase was chosen to separate the antibacterial crude diterpenes-rich sample (18.0 g) into three fractions with a recovery of 46.1%. Then, the antibacterial fractions I (200 mg), II (200 mg), and III (150 g) were separated by the Megress C18 preparative column, and compounds tanshinone IIA (80.0 mg), salvinolone (62.0 mg), cryptotanshinone (70.0 mg), and ferruginol (68.0 mg) were produced with purities greater than 98%. The procedure achieved large-scale preparation of the four diterpenes with high purity, and it could act as a reference for the efficient preparation of active diterpenes from other plant extracts.
WOS关键词	COUNTER-CURRENT CHROMATOGRAPHY ; MILTIORRHIZA-BUNGE ; TANSHINONES ; PURIFICATION ; CELLS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	MDPI
WOS记录号	WOS:000428514100114
资助机构	High and New Technology Research and Development Planning, Significant Science & Technological Project of Qinghai Province(2014-GX-A3A) ; Natural Science Funds of Qinghai Province(2016-ZJ-933Q) ; Project of Discovery, Evaluation and Transformation of Active Natural Compounds, Strategic Biological Resources Service Network Programme of Chinese Academy of Sciences(ZSTH-027) ; West Light Foundation of the Chinese Academy of Sciences for Doctors ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017471)
内容类型	期刊论文
源URL	[http://ir.nwipb.ac.cn/handle/363003/13838]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
通讯作者	Shao, Yun; Tao, Yanduo
作者单位	1.Shaanxi Univ Technol, Shaanxi Key Lab Bioresources, Hanzhong 723000, Peoples R China 2.Qinghai Prov Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China
推荐引用方式 GB/T 7714	Shao, Yun,Tao, Yanduo,Wang, Qilan,et al. Efficient Separation of Four Antibacterial Diterpenes from the Roots of Salvia Prattii Using Non-Aqueous Hydrophilic Solid-Phase Extraction Followed by Preparative High-Performance Liquid Chromatography[J]. MOLECULES,2018,23(3).
APA	Shao, Yun.,Tao, Yanduo.,Wang, Qilan.,Mei, Lijuan.,Jiao, Lijin.,...&Dang, Jun.(2018).Efficient Separation of Four Antibacterial Diterpenes from the Roots of Salvia Prattii Using Non-Aqueous Hydrophilic Solid-Phase Extraction Followed by Preparative High-Performance Liquid Chromatography.MOLECULES,23(3).
MLA	Shao, Yun,et al."Efficient Separation of Four Antibacterial Diterpenes from the Roots of Salvia Prattii Using Non-Aqueous Hydrophilic Solid-Phase Extraction Followed by Preparative High-Performance Liquid Chromatography".MOLECULES 23.3(2018).