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	Identifying egfr-expressed cells and detecting egfr multi-mutations at single-cell level by microfluidic chip
	Li, Ren1,2,5; Zhou, Mingxing1; Li, Jine1; Wang, Zihua1; Zhang, Weikai1; Yue, Chunyan1; Ma, Yan1; Peng, Hailin2; Wei, Zewen1; Hu, Zhiyuan1,3,4
刊名	Nano-micro letters
	2018
卷号	10期号:1页码:10
关键词	Egfr mutation Single-cell analysis Microfluidic chip Tyrosine kinase inhibitor
ISSN号	2311-6706
DOI	10.1007/s40820-017-0168-y
通讯作者	Wei, zewen(weizw@nanoctr.cn) ; Hu, zhiyuan(huzy@nanoctr.cn)
英文摘要	Egfr mutations companion diagnostics have been proved to be crucial for the efficacy of tyrosine kinase inhibitor targeted cancer therapies. to uncover multiple mutations occurred in minority of egfr-mutated cells, which may be covered by the noises from majority of unmutated cells, is currently becoming an urgent clinical requirement. here we present the validation of a microfluidic-chip-based method for detecting egfr multimutations at single-cell level. by trapping and immunofluorescently imaging single cells in specifically designed silicon microwells, the egfr-expressed cells were easily identified. by in situ lysing single cells, the cell lysates of egfr-expressed cells were retrieved without cross-contamination. benefited from excluding the noise from cells without egfr expression, the simple and cost-effective sanger's sequencing, but not the expensive deep sequencing of the whole cell population, was used to discover multi-mutations. we verified the new method with precisely discovering three most important egfr drugrelated mutations from a sample in which egfr-mutated cells only account for a small percentage of whole cell population. the microfluidic chip is capable of discovering not only the existence of specific egfr multi-mutations, but also other valuable single-cell-level information: on which specific cells the mutations occurred, or whether different mutations coexist on the same cells. this microfluidic chip constitutes a promising method to promote simple and cost-effective sanger's sequencing to be a routine test before performing targeted cancer therapy.
WOS关键词	GROWTH-FACTOR RECEPTOR ; LUNG-CANCER PATIENTS ; WHOLE-GENOME AMPLIFICATION ; CIRCULATING TUMOR-CELLS ; TYROSINE KINASE ; CLINICAL-RESPONSE ; GEFITINIB ; IDENTIFICATION ; CHEMOTHERAPY ; INHIBITION
WOS研究方向	Science & Technology - Other Topics ; Materials Science ; Physics
WOS类目	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000419572100003
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2177901
专题	高能物理研究所
通讯作者	Wei, Zewen; Hu, Zhiyuan
作者单位	1.Natl Ctr Nanosci & Technol China, CAS Ctr Excellence Nanosci, CAS Key Lab Standardizat & Measurement Nanotechno, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 2.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China 3.Univ Chinese Acad Sci, Sinodanish Coll, Beijing 100049, Peoples R China 4.Yangtze River Delta Acad Nanotechnol & Ind Dev Re, Jiaxing 314000, Zhejiang, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Ren,Zhou, Mingxing,Li, Jine,et al. Identifying egfr-expressed cells and detecting egfr multi-mutations at single-cell level by microfluidic chip[J]. Nano-micro letters,2018,10(1):10.
APA	Li, Ren.,Zhou, Mingxing.,Li, Jine.,Wang, Zihua.,Zhang, Weikai.,...&Hu, Zhiyuan.(2018).Identifying egfr-expressed cells and detecting egfr multi-mutations at single-cell level by microfluidic chip.Nano-micro letters,10(1),10.
MLA	Li, Ren,et al."Identifying egfr-expressed cells and detecting egfr multi-mutations at single-cell level by microfluidic chip".Nano-micro letters 10.1(2018):10.

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