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	In situ monitoring intracellular structural change of nanovehicles through photoacoustic signals based on phenylboronate-linked rgd-dextran/purpurin 18 conjugates
	Cheng, Dong-Bing1; Qi, Guo-Bin1; Wang, Jing-Qi1; Gong, Yong1; Liu, Fu-Hua1; Yu, Haijun2,3; Qiao, Zeng-Ying1; Wang, Hao1
刊名	Biomacromolecules
	2017-04-01
卷号	18期号:4页码:1249-1258
ISSN号	1525-7797
DOI	10.1021/acs.biomac.6b01922
通讯作者	Qiao, zeng-ying(qiaozy@nanoctr.cn) ; Wang, hao(wanghao@nanoctr.cn)
英文摘要	The stimuli-responsive polymeric nanocarriers have been studied extensively, and their structural changes in cells are important for the controlled intracellular drug release. the present work reported rgd-dextran/purpurin 18 conjugates with ph-responsive phenylboronate as spacer for monitoring the structural change of nanovehicles through ratiometric photoacoustic (pa) signal. phenylboronic acid modified purpurin 18 (npba-p18) could attach onto the rgd-decorated dextran (rgd-dex), and the resulting rgd-dex/npba-p18 (rdnp) conjugates with different molar ratios of rgd-dex and npba-p18 were prepared. when the moles of npba-p18 were equivalent to more than triple of rgd-dex, the single-stranded rdnp conjugates could self-assemble into nanoparticles in aqueous solution due to the fairly strong hydrophobicity of npba-p18. the ph-responsive aggregations of npba-p18 were investigated by uv-vis, fluorescence, and circular dichroism spectra, as well as transmission electron microscope. based on distinct pa signals between monomeric and aggregated state, ratiometric pa signal of i-750/i-710 could be presented to trace the structural change progress. compared with rdnp single chains, the nanoparticles exhibited effective cellular internalization through endocytosis pathway. furthermore, the nanoparticles could form well-ordered aggregates responding to intracellular acidic environment, and the resulting structural change was also monitored by ratiometric pa signal. therefore, the noninvasive pa approach could provide a deep insight into monitoring the intracellular structural change process of stimuli-responsive nanocarriers.
WOS关键词	SENSITIVE DRUG-DELIVERY ; BORONIC ACID-ESTER ; CONTROLLED-RELEASE ; POLY(BETA-AMINO ESTER)S ; BACTERIOCHLOROPHYLL C ; PH ; MICELLES ; NANOPARTICLES ; CELLS ; NANOCARRIERS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Organic ; Polymer Science
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000399061100020
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2177219
专题	高能物理研究所
通讯作者	Qiao, Zeng-Ying; Wang, Hao
作者单位	1.Natl Ctr Nanosci & Technol NCNST, Lab Biol Effects Nanomat & Nanosafety, CAS Ctr Excellence Nanosci, Beijing 100190, Peoples R China 2.Chinese Acad Sci, State Key Lab Drug Res, 501 Haike Rd, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Ctr Pharmaceut, Shanghai Inst Mat Med, 501 Haike Rd, Shanghai 201203, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Dong-Bing,Qi, Guo-Bin,Wang, Jing-Qi,et al. In situ monitoring intracellular structural change of nanovehicles through photoacoustic signals based on phenylboronate-linked rgd-dextran/purpurin 18 conjugates[J]. Biomacromolecules,2017,18(4):1249-1258.
APA	Cheng, Dong-Bing.,Qi, Guo-Bin.,Wang, Jing-Qi.,Gong, Yong.,Liu, Fu-Hua.,...&Wang, Hao.(2017).In situ monitoring intracellular structural change of nanovehicles through photoacoustic signals based on phenylboronate-linked rgd-dextran/purpurin 18 conjugates.Biomacromolecules,18(4),1249-1258.
MLA	Cheng, Dong-Bing,et al."In situ monitoring intracellular structural change of nanovehicles through photoacoustic signals based on phenylboronate-linked rgd-dextran/purpurin 18 conjugates".Biomacromolecules 18.4(2017):1249-1258.

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