| Peptide-binding induced inhibition of chemokine cxcl12 | |
| Duan, Hongyang1,2,5; Zhu, Ling1,5; Peng, Jiaxi1,5; Yang, Mo1,5; Xie, Hanyi1,2,5; Lin, Yuchen1,5; Li, Wenzhe1,2,5; Liu, Changliang1,5; Li, Xiaojin3,4; Guo, Hua3,4 | |
| 刊名 | Rsc advances
 |
| 2017 | |
| 卷号 | 7期号:34页码:21298-21307 |
| ISSN号 | 2046-2069 |
| DOI | 10.1039/c7ra01735a |
| 通讯作者 | Xu, haiyan(xuhy@pumc.edu.cn) ; Wang, chen(wangch@nanoctr.cn) ; Yang, yanlian(yangyl@nanoctr.cn) |
| 英文摘要 | The chemokine cxcl12, and its receptor cxcr4, have been recognized to be involved in various instances of cancer metastasis. the cxcl12/cxcr4 axis has emerged as a potential target for cancer therapy. here, we demonstrate a designed peptide (w4) targeting cxcl12 with high binding affinity, and describe its significant inhibitory effect on the cxcl12/cxcr4 axis. we show that w4 has comparable binding affinity (kd = 5.7 x 10(-8) m) to that of the antibody of cxcl12 (k-d = 3.0 x 10(-9) m) using the surface plasma resonance (spr) technique. upon introduction of w4, the circular dichroism (cd) spectra show that the alpha-helical structure of cxcl12 gradually transformed into a beta-sheet and random coil. these effects lead to the significant inhibitory effects on the cxcl12/cxcr4 axis using the cxcr4-positive breast cancer cell lines mcf-7 and mda-mb-231 and the leukemia cell lines hl-60 and u937 as models. the results show that w4 significantly inhibits cxcl12-induced cell migration of mcf-7, mda-mb-231, hl-60 and u937 even to 20.0% when the mole ratio is 1 : 1, completely abolishing the effect of cxcl12. these effects may provide evidence of the modulating ligand-receptor interactions of peptides as antiligand molecules that differ from the traditional receptor antagonists leading to therapeutic agents. |
| WOS关键词 | EPITHELIAL-MESENCHYMAL TRANSITION ; STROMAL-DERIVED FACTOR-1-ALPHA ; BREAST-CANCER METASTASIS ; LEUKEMIA B-CELLS ; CXCR4 ANTAGONISTS ; RECEPTOR CXCR4 ; TUMOR-CELLS ; IN-VITRO ; MIGRATION ; ACTIVATION |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Multidisciplinary |
| 语种 | 英语 |
| 出版者 | ROYAL SOC CHEMISTRY |
| WOS记录号 | WOS:000399722300068 |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2177191 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Xu, Haiyan; Wang, Chen; Yang, Yanlian |
| 作者单位 | 1.Natl Ctr Nanosci & Technol, CAS Ctr Excellence Nanosci, CAS Key Lab Standardizat & Measurement Nanotechno, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 2.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China 3.Chinese Acad Med Sci, Inst Basic Med Sci, Beijing 100005, Peoples R China 4.Peking Union Med Coll, Beijing 100005, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Duan, Hongyang,Zhu, Ling,Peng, Jiaxi,et al. Peptide-binding induced inhibition of chemokine cxcl12[J]. Rsc advances,2017,7(34):21298-21307. |
| APA | Duan, Hongyang.,Zhu, Ling.,Peng, Jiaxi.,Yang, Mo.,Xie, Hanyi.,...&Yang, Yanlian.(2017).Peptide-binding induced inhibition of chemokine cxcl12.Rsc advances,7(34),21298-21307. |
| MLA | Duan, Hongyang,et al."Peptide-binding induced inhibition of chemokine cxcl12".Rsc advances 7.34(2017):21298-21307. |
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