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	A dual-readout chemiluminescent-gold lateral flow test for multiplex and ultrasensitive detection of disease biomarkers in real samples
	Chen, Yiping1,2; Sun, Jiashu1,2; Xianyu, Yunlei1,2; Yin, Binfeng1,2; Niu, Yajing1,2; Wang, Songbai1,2,5; Cao, Fengjing1,2; Zhang, Xiaoqing1,2; Wang, Yu3,4; Jiang, Xingyu1,2
刊名	Nanoscale
	2016
卷号	8期号:33页码:15205-15212
ISSN号	2040-3364
DOI	10.1039/c6nr04017a
通讯作者	Jiang, xingyu(xingyujiang@nanoctr.cn)
英文摘要	Even though the gold lateral flow test (glft) is low-cost and allows for point-of-care testing (poct), its intrinsic limitations including low sensitivity and incapability of quantification significantly hinder the clinical application of glft for assaying disease biomarkers. to improve the performance of the glft without sacrificing its simplicity, we develop a chemiluminescent-gold lateral flow test (c-mode glft) for quantitative and multiplex detection of disease biomarkers with an ultrahigh sensitivity at a picomolar level. horseradish peroxidase (hrp) and antibody (ab) are simultaneously labeled onto the surface of gold nanoparticles (aunps) to achieve a dual-readout (chemiluminescent and visual, c&v-mode glft). a red color appears at the test line caused by the accumulation of captured aunps in the presence of targets, while hrp on the surface of aunps catalyzes the chemiluminescence reaction of luminol to amplify the signal. c-mode glft is successfully used for detecting tumor biomarkers (alpha fetoprotein, afp, and carcino embryonic antigen, cea) and bacterial infection biomarkers (procalcitonin, pct) in serum samples as well as whole blood. the excellent features of c-mode glft such as straightforward operation, ultrahigh sensitivity and quantitative detection, make it a promising platform for poct of a variety of disease biomarkers in real samples.
WOS关键词	RESONANCE ENERGY-TRANSFER ; SENSITIVE DETECTION ; IMMUNOASSAY ; BIOSENSOR ; NANOPARTICLES ; IMMUNOSENSOR ; PLATFORM ; STRIP ; ARRAY ; ASSAY
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS类目	Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000382068000015
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2176588
专题	高能物理研究所
通讯作者	Jiang, Xingyu
作者单位	1.Natl Ctr NanoSci & Technol, Beijing Engn Res Ctr BioNanotechnol, Beijing 100190, Peoples R China 2.Natl Ctr NanoSci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 3.Capital Med Univ, Beijing Inst Trop Med, 95 Yongan Rd, Beijing 100050, Peoples R China 4.Capital Med Univ, Beijing Friendship Hosp, 95 Yongan Rd, Beijing 100050, Peoples R China 5.Shanxi Univ, Res Ctr Environm Sci & Engn, Dept Chem & Chem Engn, Taiyuan 030006, Peoples R China
推荐引用方式 GB/T 7714	Chen, Yiping,Sun, Jiashu,Xianyu, Yunlei,et al. A dual-readout chemiluminescent-gold lateral flow test for multiplex and ultrasensitive detection of disease biomarkers in real samples[J]. Nanoscale,2016,8(33):15205-15212.
APA	Chen, Yiping.,Sun, Jiashu.,Xianyu, Yunlei.,Yin, Binfeng.,Niu, Yajing.,...&Jiang, Xingyu.(2016).A dual-readout chemiluminescent-gold lateral flow test for multiplex and ultrasensitive detection of disease biomarkers in real samples.Nanoscale,8(33),15205-15212.
MLA	Chen, Yiping,et al."A dual-readout chemiluminescent-gold lateral flow test for multiplex and ultrasensitive detection of disease biomarkers in real samples".Nanoscale 8.33(2016):15205-15212.

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