| Measuring binding constants of cucurbituril-based host-guest interactions at the single-molecule level with nanopores | |
| You, Yi1; Zhou, Ke2; Guo, Bingyuan2; Liu, Quansheng3; Cao, Zhong1; Liu, Lei3; Wu, Hai-Chen2 | |
| 刊名 | Acs sensors
 |
| 2019-03-01 | |
| 卷号 | 4期号:3页码:774-779 |
| 关键词 | Cucurbituril Host-guest interactions Binding constant Nanopore Dna probe |
| ISSN号 | 2379-3694 |
| DOI | 10.1021/acssensors.9b00408 |
| 通讯作者 | Cao, zhong(zhongcao2004@163.com) ; Wu, hai-chen(haichenwu@iccas.ac.cn) |
| 英文摘要 | Cucurbiturils are one type of widely used macrocyclic host compound in supramolecular chemistry. their peculiar properties have led to applications in a wide variety of research areas such as fluorescence spectroscopy, drug delivery, catalysis, and nanotechnology. however, the solubilities of cucurbiturils are rather poor in water and many organic solvents, which may cause accuracy problems when measuring binding constants with traditional methods. in this report, we aim to develop an approach to measure the binding constants of cucurbituril-based host-guest interactions at the single-molecule level. first, we covalently attach different guest compounds to the side-chain of dna molecules. then, excess cucurbiturils are incubated with dna probes to form the host-guest complexes. next, the modified dna hybrids are threaded through alpha-hemolysin nanopore to generate highly characteristic current events. finally, statistical analyses of the obtained events afford the binding constants of cucurbiturils with various molecules. this new approach provides a simple and straightforward method to compare binding strength of different host-guest complexes and may find applications for quantifying other macrocycle-based host-guest interactions. |
| 资助项目 | National Natural Science Foundation of China[31571010] ; National Natural Science Foundation of China[21475132] ; National Natural Science Foundation of China[21621062] ; Institute of Chemistry, Chinese Academy of Sciences |
| WOS关键词 | PROTEIN TRANSLOCATION ; AMINO-ACIDS ; DNA ; RECOGNITION ; CANCER ; SPECTROSCOPY ; MICRORNAS ; CHEMISTRY ; PEPTIDES ; KINETICS |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics |
| WOS类目 | Chemistry, Multidisciplinary ; Chemistry, Analytical ; Nanoscience & Nanotechnology |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000462554200030 |
| 资助机构 | National Natural Science Foundation of China ; Institute of Chemistry, Chinese Academy of Sciences |
| 内容类型 | 期刊论文 |
| URI标识 | http://www.corc.org.cn/handle/1471x/2160698 |
| 专题 | 高能物理研究所 |
| 通讯作者 | Cao, Zhong; Wu, Hai-Chen |
| 作者单位 | 1.Changsha Univ Sci & Technol, Collaborat Innovat Ctr Micro Nano Biosensing & Fo, Hunan Prov Key Lab Mat Protect Elect Power & Tran, Sch Chem & Biol Engn, Changsha 410114, Hunan, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Analyt Chem Living Biosyst, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Inst High Energy Phys, Key Lab Biomed Effects Nanomat & Nanosafety, Multidisciplinary Ctr, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | You, Yi,Zhou, Ke,Guo, Bingyuan,et al. Measuring binding constants of cucurbituril-based host-guest interactions at the single-molecule level with nanopores[J]. Acs sensors,2019,4(3):774-779. |
| APA | You, Yi.,Zhou, Ke.,Guo, Bingyuan.,Liu, Quansheng.,Cao, Zhong.,...&Wu, Hai-Chen.(2019).Measuring binding constants of cucurbituril-based host-guest interactions at the single-molecule level with nanopores.Acs sensors,4(3),774-779. |
| MLA | You, Yi,et al."Measuring binding constants of cucurbituril-based host-guest interactions at the single-molecule level with nanopores".Acs sensors 4.3(2019):774-779. |
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