Micro-systems for Optical Protein-Chip

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	Micro-systems for Optical Protein-Chip
	Jin G(靳刚); Wang ZH(王战会)
刊名	International Journal of Nonlinear Sciences and Numerical Simulation
	2002
卷号	3 期号:3-4 页码:191-194
ISSN号	1565-1339
通讯作者	Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China.
中文摘要	Protein-Chip as micro-assays for the determination of protein interaction, the analysis, the identification and the purification of proteins has large potential applications. The Optical Protein-Chip is able to detect the multi-interaction of proteins and multi-bio-activities of molecules directly and simultaneously with no labeling. The chip is a small matrix on solid substrate containing multi-micro-area prepared by microfabrication with photolithography or soft lithography for surface patterning, and processed with surface modification which includes the physical, chemical, and bio-chemical modifications, etc. The ligand immobilization, such as protein immobilization, especially the oriented immobilization with low steric hindrance and high bio-specific binding activity between ligand and receptor is used to form a sensing surface. Each area of the pattern is corresponding to only one bioactivity. The interval between the areas is non-bioactive and optically extinctive. The affinity between proteins is used to realize non-labeling microassays for the determination of protein identification and protein interaction. The sampling of the chip is non-disturbing, performed with imaging ellipsometry and image processing on a database of proteins.
学科主题	力学
类目[WOS]	Engineering, Multidisciplinary ; Mathematics, Applied ; Mechanics ; Physics, Mathematical
研究领域[WOS]	Engineering ; Mathematics ; Mechanics ; Physics
关键词[WOS]	IMAGING ELLIPSOMETRY ; VISUALIZATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000176903800007
公开日期	2007-06-15 ; 2007-12-05 ; 2009-06-23
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/17540]
专题	力学研究所_力学所知识产出(1956-2008)
推荐引用方式 GB/T 7714	Jin G,Wang ZH. Micro-systems for Optical Protein-Chip[J]. International Journal of Nonlinear Sciences and Numerical Simulation,2002,3(3-4):191-194.
APA	Jin G,&Wang ZH.(2002).Micro-systems for Optical Protein-Chip.International Journal of Nonlinear Sciences and Numerical Simulation,3(3-4),191-194.
MLA	Jin G,et al."Micro-systems for Optical Protein-Chip".International Journal of Nonlinear Sciences and Numerical Simulation 3.3-4(2002):191-194.