Developmental expression of steroidogenic factor-1, cyp19a1a and cyp19a1b from common carp (Cyprinus carpio)

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	Developmental expression of steroidogenic factor-1, cyp19a1a and cyp19a1b from common carp (Cyprinus carpio)
	Tang, Bin ; Hu, Wei ; Hao, Jun ; Zhu, Zuoyan
	2010
会议名称	2nd International Symposium for Fish Growth and Reporduction
会议日期	JUN 20-21, 2009
会议地点	Hong Kong, PEOPLES R CHINA
关键词	SF-1 cyp19a1a cyp19a1b Developmental expression Gonad development
页码	408-416
通讯作者	Hu, W, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Steroidogenic factor-1 (SF-1), cyp19a1a and cyp19a1b play pivotal roles in vertebrate steroidogenesis and reproduction. In this study, a SF-1 cDNA (EU022463) was cloned from common carp (Cyprinus carpio). The transcript contains a 1509 base pair (bp) open reading frame (ORF) encoding a 503 amino acid sequence. Comparisons of deduced amino acid sequences demonstrated that carp SF-1 is highly homologous with those of other vertebrates. Tissue specific expressions of SF-1, cyp19a1a and cyp19a1b mRNA were analyzed in 10-month-old carp. SF-1 was abundant in the hypothalamus, pituitary, gonad, spleen and liver (females only). Cyp19a1b was preferentially expressed in the brain of both sexes but also was present at much lower levels in testis, ovary and kidney (females only). Although cyp19a1a expression was preferentially expressed in ovaries, it was also present at much lower levels in brain, testis, kidney and spleen (males only). Northern blot analysis revealed that testes and brains of both sexes expressed a transcript of about 2.8 kb in size. The expression pattern of SF-1, cyp19a1a and cyp19a1b in carp gonads suggested their involvement in sexual development. In 3-month-old carp, SF-1 and cyp19a1b were expressed highly in testes but were at much lower levels in ovaries, while the opposite pattern was observed with cyp19a1a expression. In 10-month-old carp, SF-1 expression was much higher in testes than in ovaries, while the opposite pattern was observed with cyp19a1a expression. These developmental expression patterns in carp gonads suggest important roles of SF-1 and cyp19a1b in testis development and of cyp19a1a in ovary development. (C) 2010 Elsevier Inc. All rights reserved.
收录类别	SCI
会议录	GENERAL AND COMPARATIVE ENDOCRINOLOGY
会议录出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
会议录出版地	SAN DIEGO
语种	英语
ISSN号	0016-6480
WOS记录号	WOS:000278875100011
内容类型	会议论文
源URL	[http://ir.ihb.ac.cn/handle/342005/13876]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_会议论文
推荐引用方式 GB/T 7714	Tang, Bin,Hu, Wei,Hao, Jun,et al. Developmental expression of steroidogenic factor-1, cyp19a1a and cyp19a1b from common carp (Cyprinus carpio)[C]. 见:2nd International Symposium for Fish Growth and Reporduction. Hong Kong, PEOPLES R CHINA. JUN 20-21, 2009.