Shortening the breeding period of transgenic fish using growth hormone levels as an indicator

CORC > 水生生物研究所 > 鱼类生物学及渔业生物技术研究中心 > 期刊论文

	Shortening the breeding period of transgenic fish using growth hormone levels as an indicator
	Zhong, S.1,2; Wang, Y.1; Chen, Z.2; Lu, P.2; Liao, L.1; Zhu, Z.1
刊名	JOURNAL OF APPLIED ICHTHYOLOGY
	2010-12-01
卷号	26 期号:6 页码:904-907
ISSN号	0175-8659
通讯作者	Wang, Y, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China ; wangyp@ihb.ac.cn
中文摘要	In order to shorten the breeding period of fast-growing transgenic fish and reduce the workload of selection, this study introduced a new screening method to detect the growth hormone (GH) serum levels in Cyprinid fish by the combination of ELISA and monoclonal antibody technology. To test the effectiveness of this new method, the serum GH levels and growth rates between transgenic carps and controls were examined; at the same time the conventional PCR method was also employed for comparison. In the F-4-generation, which were the descendents of fast-growing transgenic or normal carps, the serum GH levels between transgenic and control carps were significantly different by ELISA. Transgenic carp growth rates were consistently and significantly higher than in the controls. Compared with the conventional PCR screening that easily resulted in false positive outcomes, the new screening method by ELISA detection directly selected the effectively integrated transgenic fishes, which can remarkably shorten the breeding period of transgenic fish.
英文摘要	In order to shorten the breeding period of fast-growing transgenic fish and reduce the workload of selection, this study introduced a new screening method to detect the growth hormone (GH) serum levels in Cyprinid fish by the combination of ELISA and monoclonal antibody technology. To test the effectiveness of this new method, the serum GH levels and growth rates between transgenic carps and controls were examined; at the same time the conventional PCR method was also employed for comparison. In the F-4-generation, which were the descendents of fast-growing transgenic or normal carps, the serum GH levels between transgenic and control carps were significantly different by ELISA. Transgenic carp growth rates were consistently and significantly higher than in the controls. Compared with the conventional PCR screening that easily resulted in false positive outcomes, the new screening method by ELISA detection directly selected the effectively integrated transgenic fishes, which can remarkably shorten the breeding period of transgenic fish.
学科主题	Fisheries; Marine & Freshwater Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Marine & Freshwater Biology
研究领域[WOS]	Fisheries ; Marine & Freshwater Biology
关键词[WOS]	BODY-COMPOSITION ; CYPRINUS-CARPIO ; GENE ; ENHANCEMENT ; PERFORMANCE ; AQUACULTURE ; TROUT
收录类别	SCI
资助信息	Chinese National Programs for High Technology Research and Development [2007AA10Z186]; National Natural Science Foundation [30771652]
语种	英语
WOS记录号	WOS:000284171500011
公开日期	2011-04-14
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/15498]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Wuhan Univ, Basic Med Sch, Dept Genet, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Zhong, S.,Wang, Y.,Chen, Z.,et al. Shortening the breeding period of transgenic fish using growth hormone levels as an indicator[J]. JOURNAL OF APPLIED ICHTHYOLOGY,2010,26(6):904-907.
APA	Zhong, S.,Wang, Y.,Chen, Z.,Lu, P.,Liao, L.,&Zhu, Z..(2010).Shortening the breeding period of transgenic fish using growth hormone levels as an indicator.JOURNAL OF APPLIED ICHTHYOLOGY,26(6),904-907.
MLA	Zhong, S.,et al."Shortening the breeding period of transgenic fish using growth hormone levels as an indicator".JOURNAL OF APPLIED ICHTHYOLOGY 26.6(2010):904-907.