Chromosomal localization of rDNA genes and genomic organization of 5S rDNA in Oreochromis mossambicus, O. urolepis hornorum and their hybrid

CORC > 水生生物研究所 > 鱼类生物学及渔业生物技术研究中心 > 期刊论文

	Chromosomal localization of rDNA genes and genomic organization of 5S rDNA in Oreochromis mossambicus, O. urolepis hornorum and their hybrid
	Zhu, Hua Ping 1; Lu, Mai Xin 1; Gao, Feng Ying 1; Huang, Zhang Han 1; Yang, Li Ping 1; Gui, Jian Fang 2
刊名	JOURNAL OF GENETICS
	2010-08-01
卷号	89 期号:2 页码:163-171
关键词	karyotype 5S rDNA NORs fluorescent in situ hybridization (FISH) tilapia
ISSN号	0022-1333
通讯作者	Lu, MX, Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China
中文摘要	In this study, classical and molecular cytogenetic analyses were performed in tilapia fishes, Oreochromis mossambicus (XX/XY sex determination system), O urolepis hornorum (WZ/ZZ sex determination system) and their hybrid by crossing O mossambicus female x O. u hornorum male. An identical karyotype ((2n = 44, NF (total number of chromosomal arms) = 50) was obtained from three examined tilapia samples. Genomic organization analysis of 5S rDNA revealed two different types of 5S rDNA sequences, 5S type I and 5S type II. Moreover, fluorescence in situ hybridization (FISH) with 5S rDNA probes showed six positive fluorescence signals on six chromosomes of all the analysed metaphases from the three tilapia samples. Subsequently, 45S rDNA probes were also prepared, and six positive fluorescence signals were observed on three chromosome pairs in all analysed metaphases of the three tilapia samples. The correlation between 45 rDNA localization and nucleolar organizer regions (NORs) was confirmed by silver nitrate staining in tilapia fishes. Further, different chromosomal localizations of 5S rDNA and 45S rDNA were verified by two different colour FISH probes. Briefly, the current data provide an insights for hybridization projects and breeding improvement of tilapias.
英文摘要	In this study, classical and molecular cytogenetic analyses were performed in tilapia fishes, Oreochromis mossambicus (XX/XY sex determination system), O urolepis hornorum (WZ/ZZ sex determination system) and their hybrid by crossing O mossambicus female x O. u hornorum male. An identical karyotype ((2n = 44, NF (total number of chromosomal arms) = 50) was obtained from three examined tilapia samples. Genomic organization analysis of 5S rDNA revealed two different types of 5S rDNA sequences, 5S type I and 5S type II. Moreover, fluorescence in situ hybridization (FISH) with 5S rDNA probes showed six positive fluorescence signals on six chromosomes of all the analysed metaphases from the three tilapia samples. Subsequently, 45S rDNA probes were also prepared, and six positive fluorescence signals were observed on three chromosome pairs in all analysed metaphases of the three tilapia samples. The correlation between 45 rDNA localization and nucleolar organizer regions (NORs) was confirmed by silver nitrate staining in tilapia fishes. Further, different chromosomal localizations of 5S rDNA and 45S rDNA were verified by two different colour FISH probes. Briefly, the current data provide an insights for hybridization projects and breeding improvement of tilapias.
学科主题	Genetics & Heredity
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Genetics & Heredity
研究领域[WOS]	Genetics & Heredity
关键词[WOS]	RIBOSOMAL-RNA GENES ; CARASSIUS-AURATUS-GIBELIO ; IN-SITU HYBRIDIZATION ; NILOTICUS GENOME ; PERCIFORMES ; CICHLIDAE ; TILAPIA ; FISHES ; DNA ; EVOLUTION
收录类别	SCI
资助信息	National Key Technology Research and Development Program [2006BAD01A1201]; Special Fund for Commonweal (Agricultural Industry) Scientific Research [3-49]; Key Program me for Agriculture Research of Guangdong Province [2009B020201003]; Natural Science Foundation of Guangdong Province [9451038001003595]; Earmarked Fund for Modern Agro-industry Technology Research System [nycytx-48-4]; Open Foundation of the State Key Laboratory of Freshwater Ecology and Biotechnolog of China [2009FB03]
语种	英语
WOS记录号	WOS:000281515700005
公开日期	2010-12-23
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/13589]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Hua Ping,Lu, Mai Xin,Gao, Feng Ying,et al. Chromosomal localization of rDNA genes and genomic organization of 5S rDNA in Oreochromis mossambicus, O. urolepis hornorum and their hybrid[J]. JOURNAL OF GENETICS,2010,89(2):163-171.
APA	Zhu, Hua Ping,Lu, Mai Xin,Gao, Feng Ying,Huang, Zhang Han,Yang, Li Ping,&Gui, Jian Fang.(2010).Chromosomal localization of rDNA genes and genomic organization of 5S rDNA in Oreochromis mossambicus, O. urolepis hornorum and their hybrid.JOURNAL OF GENETICS,89(2),163-171.
MLA	Zhu, Hua Ping,et al."Chromosomal localization of rDNA genes and genomic organization of 5S rDNA in Oreochromis mossambicus, O. urolepis hornorum and their hybrid".JOURNAL OF GENETICS 89.2(2010):163-171.