Isolation, identification and characterization of phytoplankton-lytic bacterium CH-22 against Microcystis aeruginosa

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	Isolation, identification and characterization of phytoplankton-lytic bacterium CH-22 against Microcystis aeruginosa
	Zhang, Hong 1; Yu, Zengliang 1; Huang, Qing 1; Xiao, Xiang 2; Wang, Xu 1; Zhang, Fayu 1; Wang, Xiangqin 1; Liu, Yongding 3; Hu, Chunxiang 3
刊名	LIMNOLOGICA
	2011-02-01
卷号	41 期号:1 页码:70-77
关键词	Phytoplankton-lytic bacterium Pseudomonas putida Microcystis aeruginosa Biological control Cyanobacterial blooms
ISSN号	0075-9511
通讯作者	Zhang, H, Chinese Acad Sci, Key Lab Ion Beam Bioengn, Inst Plasma Phys, Hefei 230031, Anhui, Peoples R China ; zh_elite@126.com, zlyu@ipp.ac.cn
中文摘要	A bacterial strain named CH-22 showing phytoplankton-lytic activity against bloom-forming cyanobacterium Microcystis aeruginosa was isolated from Lake Chaohu of Anhui Province, China. The isolated strain was identified as Pseudomonas putida by morphology and homology research based on 16S rDNA. The phytoplankton-lytic activity was confirmed by reduction in cell number and chlorophyll a concentration of M. aeruginosa in an infected culture for a defined period. The initial bacterial and M. aeruginosa densities affected the phytoplankton-lytic activity significantly. When the 15% (150 mu L/mL) concentration of bacterial cultures was infected, the highest phytoplankton-lytic activity reached to 98.8% after 7 days. When the initial M. aeruginosa density was less than 3 x 10(6) cells/mL, about 90.0% of chlorophyll a was removed. Obvious reduction in phycocyanin concentration in the treated M. aeruginosa suggests that isolated strain may possibly inhibit the synthesis of photosynthetic apparatus. Supernatants of bacterial cultures showed higher phytoplankton-lytic activity, suggesting that phytoplankton-lytic bacterium P. putida indirectly attacked M. aeruginosa cells by secretion of extracellular antialgal substances, which is characterized as anti-heat shock. The isolated P. putida also showed effective phytoplankton-lytic activity against a wide range of phytoplankton. These results suggest that indigenous bacteria isolated from eutrophic lake may be employed to regulate the ecological balance between the phytoplankton and bacteria, and consequently, to reduce the occurrence of cyanobacterial blooms in freshwaters. (C) 2010 Elsevier GmbH. All rights reserved.
英文摘要	A bacterial strain named CH-22 showing phytoplankton-lytic activity against bloom-forming cyanobacterium Microcystis aeruginosa was isolated from Lake Chaohu of Anhui Province, China. The isolated strain was identified as Pseudomonas putida by morphology and homology research based on 16S rDNA. The phytoplankton-lytic activity was confirmed by reduction in cell number and chlorophyll a concentration of M. aeruginosa in an infected culture for a defined period. The initial bacterial and M. aeruginosa densities affected the phytoplankton-lytic activity significantly. When the 15% (150 mu L/mL) concentration of bacterial cultures was infected, the highest phytoplankton-lytic activity reached to 98.8% after 7 days. When the initial M. aeruginosa density was less than 3 x 10(6) cells/mL, about 90.0% of chlorophyll a was removed. Obvious reduction in phycocyanin concentration in the treated M. aeruginosa suggests that isolated strain may possibly inhibit the synthesis of photosynthetic apparatus. Supernatants of bacterial cultures showed higher phytoplankton-lytic activity, suggesting that phytoplankton-lytic bacterium P. putida indirectly attacked M. aeruginosa cells by secretion of extracellular antialgal substances, which is characterized as anti-heat shock. The isolated P. putida also showed effective phytoplankton-lytic activity against a wide range of phytoplankton. These results suggest that indigenous bacteria isolated from eutrophic lake may be employed to regulate the ecological balance between the phytoplankton and bacteria, and consequently, to reduce the occurrence of cyanobacterial blooms in freshwaters. (C) 2010 Elsevier GmbH. All rights reserved.
学科主题	Limnology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Limnology
研究领域[WOS]	Marine & Freshwater Biology
关键词[WOS]	APHANIZOMENON-FLOS-AQUAE ; HARMFUL ALGAL BLOOMS ; BLUE-GREEN-ALGA ; TAIHU LAKE ; CYANOBACTERIAL BLOOMS ; LOCAL SOILS ; ALGICIDAL ACTIVITY ; CLAY FLOCCULATION ; WATER ; REMOVAL
收录类别	SCI
资助信息	President Foundation of Hefei Institutes of Physical Science ; Chinese Academy of Sciences [075AH24581]; National Basic Research Program of China [2007CB936602]
语种	英语
WOS记录号	WOS:000286909300009
公开日期	2011-04-14
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/15536]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Key Lab Ion Beam Bioengn, Inst Plasma Phys, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Sch Chem, Lab Environm Biotechnol, Hefei 230026, Anhui, Peoples R China 3.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Hong,Yu, Zengliang,Huang, Qing,et al. Isolation, identification and characterization of phytoplankton-lytic bacterium CH-22 against Microcystis aeruginosa[J]. LIMNOLOGICA,2011,41(1):70-77.
APA	Zhang, Hong.,Yu, Zengliang.,Huang, Qing.,Xiao, Xiang.,Wang, Xu.,...&Hu, Chunxiang.(2011).Isolation, identification and characterization of phytoplankton-lytic bacterium CH-22 against Microcystis aeruginosa.LIMNOLOGICA,41(1),70-77.
MLA	Zhang, Hong,et al."Isolation, identification and characterization of phytoplankton-lytic bacterium CH-22 against Microcystis aeruginosa".LIMNOLOGICA 41.1(2011):70-77.