题名矿质营养对黄芪生长发育及有效成分积累的影响
学位类别硕士
答辩日期2008
授予单位中国科学院研究生院
授予地点陕西
导师徐福利
学位专业生态学
中文摘要本论文以根类中药材黄芪为研究对象,采用田间试验与室内分析相结合的方法,研究了氮磷钾对膜荚黄芪生长发育及有效成分的影响,建立了膜荚黄芪氮磷钾优化施肥模式。主要研究结论如下:1膜荚黄芪多糖随着生育期的推进呈递增趋势,总黄酮、甲苷随生育期的推进逐渐增加,到816达到峰值,随后逐渐降低。幼苗期、旺盛生长期是黄芪多糖含量累积两个关键时期;旺盛生长期是黄芪总黄酮、甲苷累积的关键时期。膜荚黄芪有效成分的累积高峰滞后于干物质累积的累积高峰。

2 合理施肥促进了植株对氮磷钾的吸收积累。膜荚黄芪不同生长时期对氮、磷、钾的吸收量不同,幼苗期、旺盛生长期对氮肥需求量较大,根系膨大期对钾肥的需求量较大。氮磷钾在膜荚黄芪不同器官的含量、吸收积累量及分配比率随着生育时期发生着变化。膜荚黄芪对氮磷钾的吸收积累量随着生育期的推进逐渐增加,至816达到最高,随后呈递减趋势。植株氮磷钾浓度随着生育期的推进小幅升高,至614后呈递减趋势。幼苗期、旺盛生长期氮磷钾主要集中在茎叶中,生育后期氮磷钾主要集中在根系中,这种变化主要是因为膜荚黄芪生长中心的转移。本研究表明,膜荚黄芪植株干物质积累不仅与氮磷钾的吸收积累总量有关,还随氮磷钾的养分平衡指数直线增加。

3施肥促进了膜荚黄芪幼苗的生长,从而为生育后期膜荚黄芪根生长、产量形成及有效成分的累积提供充足的营养基础。氮、磷、钾各元素及其配比明显促进了茎叶及根干物质积累,氮、磷、钾对膜荚黄芪干物质累积总量的影响程度大小依次为氮>>磷;对膜荚黄芪茎叶干物质累积量的影响程度大小依次为氮>>钾;对根干物质累积量的影响程度大小依次为氮>>磷。施肥明显提高了膜荚黄芪根产量,氮、磷、钾对根产量影响程度大小依次为氮>>磷。氮、磷、钾各元素及其配比使黄芪多糖、黄芪甲苷含量明显增加,对总黄酮含量影响不明显。氮、磷、钾对黄芪多糖含量影响程度大小依次为>>;对黄芪甲苷含量的影响程度大小依次为氮>>磷。氮、钾对膜荚黄芪生长发育,产量形成及多糖,黄芪甲苷含量有重要的影响。

4建立了氮磷钾的施肥量编码值与膜荚黄芪根产量、多糖含量的效应函数。结果表明:氮、磷、钾肥对膜荚黄芪根产量的增产作用大小依次为:氮肥>钾肥>磷肥;氮、磷、钾肥对黄芪多糖含量的作用大小依次为:钾肥>磷肥>氮肥,其中钾肥为负效应,氮肥、磷肥为正效应。寻优结果表明,膜荚黄芪目标产量在60007000 kg/hm2之间,95%置信区间的优化施肥量为:N 66.85102.92 kg/hm2P2O5 64.6494.95 kg/hm2K2O 119.78166.48 kg/hm2;膜荚黄芪多糖含量在13%14%之间,95%置信区间的优化施肥量为:N 66.85102.92 kg/hm2P2O5 64.6494.95 kg/hm2K2O 119.78166.48 kg/hm2;膜荚黄芪高产优质高效栽培优化施肥量为:N 99.52102.92 kg/hm2P2O5 94.2094.95 kg/hm2K2O 119.78166.48 kg/hm2NP2O5K2O的最佳比例为:1:0.920.95:1.161.62

5采用主成分分析法,研究了膜荚黄芪的多糖、甲苷、总黄酮含量与全氮、全磷、全钾、铁、锰、铜、锌含量及气象因子动态变化的关系,结果表明:不同指标之间存在一定的相互促进或拮抗作用。多糖与甲苷PMn呈显著正相关,相关系数分别为0.9510.926。多糖与月平均气温、月日照时数;甲苷与月平均气温、月日照时数、Mn;总黄酮与NKCu呈显著负相关,相关系数分别为-0.956-0.934-0.950-0.912-0.892-0.957-0.884影响黄土区膜荚黄芪有效成分含量动态变化的3个因子包括:高温强照射因子、酶促反应因子、降雨因子。其中高温强照射、酶促反应因子对膜荚黄芪有效成分影响较大。
英文摘要The root drug, Astragalus membranaceus was targeted in this experiment. The effects of nitrogen, phosphorus and potassium application on growth and active ingredient of Astragalus membranaceus was studied in detail in this experiment with field experiment combined with laboratory chemical analysis. The optimization models of nitrogen, phosphorus and potassium application for Astragalus membranaceus production were established, following results were concluded:

1 With the passing of growth period, The content of polysaccharide increased progressively; The content of total flavonoids and astragaloside increased gradually, until 16th August. It had a accumulation peak, then decreased gradually. Seedling, vigorous period were key periods to accumulation of polysaccharide; Vigorous periodwas key period to accumulation of total flavonoids and astragaloside.The accumulation peak of active ingredient was lagging behind the accumulation of dry matter.

2 Fertilization promoted the absorption and accumulation of nitrogen, phosphorus and potassium. The absorptive capacity of nitrogen, phosphorus and potassium varied among different developing stages. It needed more nitrogen fertilizer at seedling, vigorous period; more potassium fertilizer at root filling period. With the passing of growth period, the content of nitrogen, phosphorus and potassium of different organs, absorption of nitrogen, phosphorus and potassium, distribution ratio of nitrogen, phosphorus and potassium was changing. With the passing of growth period, the absorption of nitrogen, phosphorus and potassium increased gradually, until 16th august it had a highest absorption, then decreased gradually; the content of nitrogen, phosphorus and potassium increased slowly, until 14th June reached the maximum, then decreased gradually. Because of the growth center transporting, most of nitrogen, phosphorus and potassium concentrate on stem-leaf at seedling, vigorous period; most of them concentrate on root at late growth period. The results showed that dry matter accumulation presented a linear increase tendency with the increasing of the total accumulative amount of nitrogen, phosphorus and potassium, and nutrient balance index .

3 Fertilization promoted the growth on seedling and provided abundant supply of nutrition for growth of root, yield and accumulation of active ingredient at later growth stage. Different proportional with nitrogen, phosphorus and potassium application promoted the accumulation of dry matter of stem-leaf and root, the effects of nitrogen, phosphorus and potassium application on the total dry matter accumulation of Astragalus membranaceus was as follows nitrogen>potassium>phosphorus; the effects on the stem-leaf dry matter accumulation was as follows nitrogen>phosphorus> potassium; the effects on the root dry matter accumulation was as follows nitrogen>potassium>phosphorus. Nitrogen, phosphorus and potassium fertilizer increased the root yield of Astragalus membranaceus. Obviously, the effects on the root yield was as follows nitrogen>potassium>phosphorus. The application of different proportional with nitrogen, phosphorus and potassium increased the content of polysaccharide and atragaloside, but had no distinct effect on the content of total flavonoids. The effects on the content of polysaccharide was as follows potassium>phosphorus>nitrogen, but the effects on the content of atragaloside was as follows nitrogen>potassium>phosphorus. Nitrogen and potassium fertilizer application had more important effect on growth, yeild and the contents of polysaccharide and atragaloside of Astragalus membranaceus.

4 The effect functions of nitrogen, phosphorus and potassium application code on root yield and polysaccharide content of Astragalus membranaceus were established based on field experiment using three factor D-saturation optimal design. The results showed that the order of the effect of nitrogen, phosphorus and potassium on yield improvement and polysaccharide was nitrogen>potassium>phosphorus and potassium>phosphorus>nitrogen, respectively. The effect of potassium on polysaccharide was negative, but the effects of nitrogen and phosphorus were positive. When the target yield was over 60007000 kg/ha, and confidence interval was 95%, the optimum fertilizer application rate was 66.85102.92 kg/ha, 64.6494.95 kg/ha, and 119.78166.48 kg/ha for N, P2O5, and K2O, respectively. When the polysaccharide content was over 13%14%, and confidence interval was 95%, the optimum fertilizer application rate was 66.85102.92kg/ha,  64.6494.95 kg/ha, and 119.78166.48 kg/ha for N, P2O5, and K2O, respectively. The optimum fertilizer application rate for high yield, efficiency and quality in Astragalus membranaceus production was 99.52102.92 kg/ha, 94.2094.95 kg/ha, and 119.78166.48 kg/ha for N, P2O5, and K2O, respectively. The optimum ratio of N:P2O5:K2O was 1:0.920.95:1.161.62.

5 By determining the content of polysaccharide, atragaloside, total flavonoids, N, P, K, Fe, Mn, Cu, Zn of Astragalus membranaceus, by analyzing the principal components, the relationship laws among the content of active ingredient, mineral elements and meteorological factors are investigated. The results showed that there was mutual promotion or antagonism between different indexes. The correlation coefficient between polysaccharide and atragaloside; P and Mn are 0.951, 0.926, respectively, their correlation is markedly in the positive 0.05 levels. The correlation coefficient between polysaccharide and monthly average temperature; polysaccharide and monthly average sunlight hours; polysaccharide and Mn; total flavonoids and N; total flavonoids and K; total flavonoids and Cu are -0.956,-0.934,-0.950,-0.912,-0.892,-0.957,-0.884, respectively, their correlation is markedly in the negative 0.05 levels. The principal factors affecting the content of active ingredient of Astragalus membranaceus were:  the factors of high temperature and sunshines; the factor of promoting enzyme; the factor of precipitation. The factors of high temperature and sunshines and promoting enzyme had important influence on active ingredient of Astragalus membranaceus.
公开日期2011-07-01
内容类型学位论文
源URL[http://ir.iswc.ac.cn/handle/361005/4102]  
专题水土保持研究所_水保所知识产出(1956-2013)
推荐引用方式
GB/T 7714
. 矿质营养对黄芪生长发育及有效成分积累的影响[D]. 陕西. 中国科学院研究生院. 2008.
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