Raman Spectroscopic Analysis of a Desert Cyanobacterium Nostoc sp in Response to UVB Radiation

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	Raman Spectroscopic Analysis of a Desert Cyanobacterium Nostoc sp in Response to UVB Radiation
	Wang, Gaohong 1; Hao, Zongjie 1,2; Huang, Zebo 3; Chen, Lanzhou 4; Li, Xiaoyan 1,2; Hu, Chunxiang 1; Liu, Yongding 1
刊名	ASTROBIOLOGY
	2010-10-01
卷号	10 期号:8 页码:783-788
关键词	Nostoc sp. UVB Raman spectra beta-carotene
ISSN号	1531-1074
通讯作者	Liu, YD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Cyanobacteria are capable of tolerating environmental extremes. To survive in extreme environments, cyanobacteria have developed the capability to adapt to a variety of stresses. For example, cyanobacteria have adopted a number of strategies with which to survive UV stress, including expression of UV-screening pigments and antioxidant systems. We have previously shown that several antioxidants are significantly expressed in Nostoc sp. by UVB irradiation. We report here that the content of UV-responsive biomarkers such as beta-carotene and scytonemin can be easily detected by Fourier transform Raman spectroscopy with use of a small sample size and that the content of beta-carotene is dependant on the UVB intensity and exposure time. Our results indicate that Raman spectroscopy may be a helpful tool to analyze UV-protective molecules of cyanobacterium in astrobiological studies without access to large sample sizes and complicated extractions, which are needed by other analytical techniques such as high-performance liquid chromatography and mass spectrometry.
英文摘要	Cyanobacteria are capable of tolerating environmental extremes. To survive in extreme environments, cyanobacteria have developed the capability to adapt to a variety of stresses. For example, cyanobacteria have adopted a number of strategies with which to survive UV stress, including expression of UV-screening pigments and antioxidant systems. We have previously shown that several antioxidants are significantly expressed in Nostoc sp. by UVB irradiation. We report here that the content of UV-responsive biomarkers such as beta-carotene and scytonemin can be easily detected by Fourier transform Raman spectroscopy with use of a small sample size and that the content of beta-carotene is dependant on the UVB intensity and exposure time. Our results indicate that Raman spectroscopy may be a helpful tool to analyze UV-protective molecules of cyanobacterium in astrobiological studies without access to large sample sizes and complicated extractions, which are needed by other analytical techniques such as high-performance liquid chromatography and mass spectrometry.
学科主题	Astronomy & Astrophysics; Biology; Geosciences, Multidisciplinary
WOS标题词	Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine
类目[WOS]	Astronomy & Astrophysics ; Biology ; Geosciences, Multidisciplinary
研究领域[WOS]	Astronomy & Astrophysics ; Life Sciences & Biomedicine - Other Topics ; Geology
关键词[WOS]	ULTRAVIOLET-B RADIATION ; OXIDATIVE DAMAGE ; EXOGENOUS ANTIOXIDANTS ; SYNECHOCOCCUS PCC7942 ; SCREENING PIGMENT ; ALGAL CRUSTS ; NITRIC-OXIDE ; CHROOCOCCIDIOPSIS ; PHOTOSYNTHESIS ; ADAPTATION
收录类别	SCI
资助信息	Natural Science Foundation of China [30400093, 30970688]; National Major Programs of Water Body Pollution Control and Remediation [2008ZX07105-005]
语种	英语
WOS记录号	WOS:000284397800002
公开日期	2010-12-23
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/13761]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Wuhan Univ, Coll Pharm, Wuhan 430072, Peoples R China 4.Wuhan Univ, Coll Resource & Environm Sci, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Wang, Gaohong,Hao, Zongjie,Huang, Zebo,et al. Raman Spectroscopic Analysis of a Desert Cyanobacterium Nostoc sp in Response to UVB Radiation[J]. ASTROBIOLOGY,2010,10(8):783-788.
APA	Wang, Gaohong.,Hao, Zongjie.,Huang, Zebo.,Chen, Lanzhou.,Li, Xiaoyan.,...&Liu, Yongding.(2010).Raman Spectroscopic Analysis of a Desert Cyanobacterium Nostoc sp in Response to UVB Radiation.ASTROBIOLOGY,10(8),783-788.
MLA	Wang, Gaohong,et al."Raman Spectroscopic Analysis of a Desert Cyanobacterium Nostoc sp in Response to UVB Radiation".ASTROBIOLOGY 10.8(2010):783-788.