Limitations of using extracellular alkaline phosphatase activities as a general indicator for describing P deficiency of phytoplankton in Chinese shallow lakes

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水环境工程研究中心 > 期刊论文

	Limitations of using extracellular alkaline phosphatase activities as a general indicator for describing P deficiency of phytoplankton in Chinese shallow lakes
	Cao, Xiuyun; Song, Chunlei; Zhou, Yiyong
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2010-02-01
卷号	22 期号:1 页码:33-41
关键词	Phytoplankton P deficiency Indicator Soluble reactive phosphorus (SRP) Alkaline phosphatase ELF Eutrophication
ISSN号	0921-8971
通讯作者	Song, CL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要	Extracellular phosphatase can be produced by phytoplankton to utilize organic phosphorus under phosphorus (P) deficiency. However, there is a controversy about its use as an indicator of P deficiency in natural phytoplankton community inferred by such an "induction-repression" mechanism. Size-fractionation of alkaline phosphatase activity (APA), soluble reactive phosphorus (SRP) concentration, algal density, and composition were determined in six Chinese shallow lakes ranking in gradient of trophic status, where a positive relationship between SRP concentration and algal density was observed. Enzyme-labeled fluorescence (ELF) method was used to localize phosphatase on cell membrane of algae. The so-called algal APA that associated with coarser particle (> 3.0 A mu m) accounted for the largest part of total APA. Within a lake with lower SRP concentration, the "induction-repression" mechanism held true. Contrastingly, both algal and total APA were positively related to SRP concentration based on the data across all study lakes with statistical significance, which may be explained firstly by algal composition. The lakes with higher SRP concentration were dominated by diatoms and green algae, while they easily produced extracellular phosphatases as evidenced by ELFA labeling. In parallel, the lakes with lower SRP concentration were dominated by cyanobacteria, while it was never ELFA-positive; secondly, ELFA-positive dots or structures suggested that, in lakes with higher trophic status, attached bacteria or heterotrophic microorganisms could substantially contribute to extracellular phosphatases for hydrolyzing organophosphoric compounds but probably utilizing the organic moiety as an organic carbon source. This process simultaneously produces inorganic P, leading to the co-occurrence of high phosphate concentration and APA. So, the contributor of APA are complex, which may produce extracellular phosphatase species-specific or not exclusively for P nutrient and consequently make it difficult to normalize APA with the exact biomass estimators. Therefore, it is not reasonable to use APA, normalized or not, as a general indicator for describing P deficiency of phytoplankton in shallow lakes especially eutrophic ones.
英文摘要	Extracellular phosphatase can be produced by phytoplankton to utilize organic phosphorus under phosphorus (P) deficiency. However, there is a controversy about its use as an indicator of P deficiency in natural phytoplankton community inferred by such an "induction-repression" mechanism. Size-fractionation of alkaline phosphatase activity (APA), soluble reactive phosphorus (SRP) concentration, algal density, and composition were determined in six Chinese shallow lakes ranking in gradient of trophic status, where a positive relationship between SRP concentration and algal density was observed. Enzyme-labeled fluorescence (ELF) method was used to localize phosphatase on cell membrane of algae. The so-called algal APA that associated with coarser particle (> 3.0 A mu m) accounted for the largest part of total APA. Within a lake with lower SRP concentration, the "induction-repression" mechanism held true. Contrastingly, both algal and total APA were positively related to SRP concentration based on the data across all study lakes with statistical significance, which may be explained firstly by algal composition. The lakes with higher SRP concentration were dominated by diatoms and green algae, while they easily produced extracellular phosphatases as evidenced by ELFA labeling. In parallel, the lakes with lower SRP concentration were dominated by cyanobacteria, while it was never ELFA-positive; secondly, ELFA-positive dots or structures suggested that, in lakes with higher trophic status, attached bacteria or heterotrophic microorganisms could substantially contribute to extracellular phosphatases for hydrolyzing organophosphoric compounds but probably utilizing the organic moiety as an organic carbon source. This process simultaneously produces inorganic P, leading to the co-occurrence of high phosphate concentration and APA. So, the contributor of APA are complex, which may produce extracellular phosphatase species-specific or not exclusively for P nutrient and consequently make it difficult to normalize APA with the exact biomass estimators. Therefore, it is not reasonable to use APA, normalized or not, as a general indicator for describing P deficiency of phytoplankton in shallow lakes especially eutrophic ones.
学科主题	Biotechnology & Applied Microbiology; Marine & Freshwater Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
关键词[WOS]	FRESH-WATER PHYTOPLANKTON ; SPRING PHYTOPLANKTON ; EUTROPHIC LAKE ; PHOSPHORUS DEFICIENCY ; ENZYME-ACTIVITY ; ORGANIC-MATTER ; RESERVOIR ; BACTERIA ; AMINOPEPTIDASE ; AVAILABILITY
收录类别	SCI
资助信息	National Science Foundation of China [2008CB418006, 30600088]; Chinese Academy of Sciences [KZCX2-YW-426]
语种	英语
WOS记录号	WOS:000274435300004
公开日期	2010-12-23
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/13725]
专题	水生生物研究所_水环境工程研究中心_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Cao, Xiuyun,Song, Chunlei,Zhou, Yiyong. Limitations of using extracellular alkaline phosphatase activities as a general indicator for describing P deficiency of phytoplankton in Chinese shallow lakes[J]. JOURNAL OF APPLIED PHYCOLOGY,2010,22(1):33-41.
APA	Cao, Xiuyun,Song, Chunlei,&Zhou, Yiyong.(2010).Limitations of using extracellular alkaline phosphatase activities as a general indicator for describing P deficiency of phytoplankton in Chinese shallow lakes.JOURNAL OF APPLIED PHYCOLOGY,22(1),33-41.
MLA	Cao, Xiuyun,et al."Limitations of using extracellular alkaline phosphatase activities as a general indicator for describing P deficiency of phytoplankton in Chinese shallow lakes".JOURNAL OF APPLIED PHYCOLOGY 22.1(2010):33-41.