Molecular analysis, developmental function and heavy metal-induced expression of ABCC5 in zebrafish

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Molecular analysis, developmental function and heavy metal-induced expression of ABCC5 in zebrafish
	Long, Yong 1,2; Li, Qing 1; Li, Jie 1,2; Cui, Zongbin 1
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
	2011
卷号	158 期号:1 页码:46-55
关键词	ABC transporter ABCC5 Heavy metals Embryogenesis Tissue defense
ISSN号	1096-4959
通讯作者	Cui, ZB, Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organism, 7 Donghu Rd, Wuhan 430072, Hubei, Peoples R China ; zbcui@ihb.ac.cn
中文摘要	ABCC5/MRP5 is an organic anion transporter that participates in tissue defense and cellular signal transduction through efflux of anticancer drugs, toxicants and a second messenger cGMP, but its physiological functions in zebrafish remain to be defined. Herein, we report the characterization, spatiotemporal expression and developmental function of zebrafish ABCC5 and its transcriptional responses to heavy metals. Zebrafish abcc5 gene is located on chromosome 18 and comprised of 28 exons. The deduced polypeptide of zebrafish ABCC5 consists of 1426 amino acids, which shares high sequence identity with those from other species. Zebrafish abcc5 is maternally expressed and its transcripts are mainly distributed in brain, lens, liver and intestine of developing embryos. In adults, zebrafish abcc5 is extensively expressed, at higher levels in testis, brain, eye, ovary, intestine and kidney, but at relatively lower levels in gill, liver, heart and muscle. Blockage of endogenous ABCC5 activity by its dominant-negative led to the developmental retardation of zebrafish embryos in which activity of p21 signaling was markedly stimulated and cellular cGMP content was significantly increased. In addition, expression of abcc5 in ZF4 cells and zebrafish embryos was significantly induced by cadmium (Cd), lead (Pb), mercury (Hg) or arsenic (As). The induced expression of ABCC5 by heavy metals was mainly detected in the liver of embryos at 96-h post-fertilization (hpf). In adult zebrafish, expression of abcc5 in brain, intestine, liver, kidney and ovary was significantly induced by one or more of these heavy metals. Furthermore, overexpression of ABCC5 attenuated the toxicity of Cd to zebrafish embryos, but did not affect the toxicity of Hg or As. Thus, ABCC5 is likely to play an active role in embryonic development and heavy metal detoxification through the export of second messenger molecules and toxicants out of cells in zebrafish. (C) 2010 Elsevier Inc. All rights reserved.
英文摘要	ABCC5/MRP5 is an organic anion transporter that participates in tissue defense and cellular signal transduction through efflux of anticancer drugs, toxicants and a second messenger cGMP, but its physiological functions in zebrafish remain to be defined. Herein, we report the characterization, spatiotemporal expression and developmental function of zebrafish ABCC5 and its transcriptional responses to heavy metals. Zebrafish abcc5 gene is located on chromosome 18 and comprised of 28 exons. The deduced polypeptide of zebrafish ABCC5 consists of 1426 amino acids, which shares high sequence identity with those from other species. Zebrafish abcc5 is maternally expressed and its transcripts are mainly distributed in brain, lens, liver and intestine of developing embryos. In adults, zebrafish abcc5 is extensively expressed, at higher levels in testis, brain, eye, ovary, intestine and kidney, but at relatively lower levels in gill, liver, heart and muscle. Blockage of endogenous ABCC5 activity by its dominant-negative led to the developmental retardation of zebrafish embryos in which activity of p21 signaling was markedly stimulated and cellular cGMP content was significantly increased. In addition, expression of abcc5 in ZF4 cells and zebrafish embryos was significantly induced by cadmium (Cd), lead (Pb), mercury (Hg) or arsenic (As). The induced expression of ABCC5 by heavy metals was mainly detected in the liver of embryos at 96-h post-fertilization (hpf). In adult zebrafish, expression of abcc5 in brain, intestine, liver, kidney and ovary was significantly induced by one or more of these heavy metals. Furthermore, overexpression of ABCC5 attenuated the toxicity of Cd to zebrafish embryos, but did not affect the toxicity of Hg or As. Thus, ABCC5 is likely to play an active role in embryonic development and heavy metal detoxification through the export of second messenger molecules and toxicants out of cells in zebrafish. (C) 2010 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Zoology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Zoology
研究领域[WOS]	Biochemistry & Molecular Biology ; Zoology
关键词[WOS]	MULTIDRUG-RESISTANCE PROTEINS ; NITRIC-OXIDE ; CYCLIC-NUCLEOTIDES ; CELL-CYCLE ; TRANSPORT ; CADMIUM ; CGMP ; MRP5 ; ACTIVATION ; BINDING
收录类别	SCI
资助信息	National Natural Science Foundation of China [30671602, 30871442]
语种	英语
WOS记录号	WOS:000286027000005
公开日期	2011-04-14
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/15528]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organism, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Long, Yong,Li, Qing,Li, Jie,et al. Molecular analysis, developmental function and heavy metal-induced expression of ABCC5 in zebrafish[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2011,158(1):46-55.
APA	Long, Yong,Li, Qing,Li, Jie,&Cui, Zongbin.(2011).Molecular analysis, developmental function and heavy metal-induced expression of ABCC5 in zebrafish.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,158(1),46-55.
MLA	Long, Yong,et al."Molecular analysis, developmental function and heavy metal-induced expression of ABCC5 in zebrafish".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 158.1(2011):46-55.