Enhanced hyperplasia in muscles of transgenic zebrafish expressing Follistatin1

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Enhanced hyperplasia in muscles of transgenic zebrafish expressing Follistatin1
	Li Xi; Nie Fen; Yin Zhan; He JiangYan
刊名	SCIENCE CHINA-LIFE SCIENCES
	2011-02-01
卷号	54 期号:2 页码:159-165
关键词	Follistatin1 myostatin muscle growth transgenic zebrafish
ISSN号	1674-7305
通讯作者	He, JY, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; jyhe@ihb.ac.cn
中文摘要	Myostatin is a member of the transforming growth factor-beta (TGF-beta) super-family and functions as a negative regulator of muscle growth. Binding of the specific receptor, Activin receptor IIB (Act RIIB), with myostatin or other related TGF-beta members, could be inhibited by the activin-binding protein follistatin (Fst) in mammals. Overexpressing Fst in mouse skeletal muscle leads to muscle hypertrophy and hyperplasia. To determine if Fst has similar roles in fish, we generated transgenic zebrafish expressing high levels of zebrafish Fst1 using the promoter of the zebrafish skeletal muscle-specific gene, myosin, light polypeptide 2, skeletal muscle (Mylz2). Independent transgenic zebrafish lines exhibited elevated expression levels of myogenic regulatory genes MyoD and Pax7 in muscle cells. Adult Fst1 overexpressing transgenic zebrafish exhibited a slight body weight increase. The high level of Fst1 expression dramatically increased myofiber numbers in skeletal muscle, without significantly changing the fiber size. Our findings suggest that Fst1 overexpression can promote zebrafish muscle growth by enhancing myofiber hyperplasia.
英文摘要	Myostatin is a member of the transforming growth factor-beta (TGF-beta) super-family and functions as a negative regulator of muscle growth. Binding of the specific receptor, Activin receptor IIB (Act RIIB), with myostatin or other related TGF-beta members, could be inhibited by the activin-binding protein follistatin (Fst) in mammals. Overexpressing Fst in mouse skeletal muscle leads to muscle hypertrophy and hyperplasia. To determine if Fst has similar roles in fish, we generated transgenic zebrafish expressing high levels of zebrafish Fst1 using the promoter of the zebrafish skeletal muscle-specific gene, myosin, light polypeptide 2, skeletal muscle (Mylz2). Independent transgenic zebrafish lines exhibited elevated expression levels of myogenic regulatory genes MyoD and Pax7 in muscle cells. Adult Fst1 overexpressing transgenic zebrafish exhibited a slight body weight increase. The high level of Fst1 expression dramatically increased myofiber numbers in skeletal muscle, without significantly changing the fiber size. Our findings suggest that Fst1 overexpression can promote zebrafish muscle growth by enhancing myofiber hyperplasia.
学科主题	Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biology
研究领域[WOS]	Life Sciences & Biomedicine - Other Topics
关键词[WOS]	DANIO-RERIO ; MYOSTATIN ; GENE ; GROWTH ; OVEREXPRESSION ; TELEOSTS ; DEFECTS ; MICE
收录类别	SCI
资助信息	National Natural Science Foundation of China [30671600]; National Basic Research Program of China [2010CB126302, 2009CB118701]
语种	英语
WOS记录号	WOS:000287702200008
公开日期	2011-04-14
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/15520]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Li Xi,Nie Fen,Yin Zhan,et al. Enhanced hyperplasia in muscles of transgenic zebrafish expressing Follistatin1[J]. SCIENCE CHINA-LIFE SCIENCES,2011,54(2):159-165.
APA	Li Xi,Nie Fen,Yin Zhan,&He JiangYan.(2011).Enhanced hyperplasia in muscles of transgenic zebrafish expressing Follistatin1.SCIENCE CHINA-LIFE SCIENCES,54(2),159-165.
MLA	Li Xi,et al."Enhanced hyperplasia in muscles of transgenic zebrafish expressing Follistatin1".SCIENCE CHINA-LIFE SCIENCES 54.2(2011):159-165.