A normal mucin-binding lectin from the sponge Craniella australiensis

	A normal mucin-binding lectin from the sponge Craniella australiensis
	Xiong, CN ; Li, W ; Liu, H ; Zhang, W ; Dou, JL ; Bai, XF ; Du, YG ; Ma, XJ
刊名	comparative biochemistry and physiology c-toxicology & pharmacology
	2006-05-01
卷号	143 期号:1 页码:9-16
关键词	sponge Craniella australiensis mucin-binding lectin glycoproteins mitogenic
通讯作者	李伟
产权排序	1;1
英文摘要	a lectin, craniella australiensis (cal), was isolated from sponge c. australiensis by ion-exchange on deae-sephacel and purified by gel filtration on sephadex g-150 and hplc on deaf-5pw. the purified lectin was a trimeric protein as revealed by sds-page and maldi-tof analysis. sds-page showed that the cal protein had a molecular mass of 54 kda, and consisted of three 18 kda subunits. gel filtration of purified lectin on sephadex g-200 indicates that it exists as a 54 kda protein in its native state. the amino acid composition was rich in thr and glx. cal was found to agglutinate native and trypsinized human a, b erythrocytes, and agglutinate native erythrocytes of mouse, sheep, rabbit and chicken, and trypsinized erythrocytes of sheep and rabbit. the hemagglutination activity was inhibited by glycoproteins such as psm and asialo-psm, but not by any of the monosaccharides tested. the activity was stable between 20 and 70 c. significant cal activity was observed between ph 5 and 8. the lectin reaction is independent of the presence of divalent cations ca2+ and mg2+. the sequence of n-terminal residues of cal was determined as tsscqsivve. the lectin showed a potent mitogenic response towards balb/c splenocytes. (c) 2005 elsevier inc. all rights reserved.
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemistry & molecular biology ; endocrinology & metabolism ; toxicology ; zoology
研究领域[WOS]	biochemistry & molecular biology ; endocrinology & metabolism ; toxicology ; zoology
关键词[WOS]	ascidian didemnum-ternatanum ; axinella-polypoides schmidt ; amino-acid-sequence ; geodia-cydonium ; human-lymphocytes ; self recognition ; horseshoe-crab ; purification ; molecules ; mitogen
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000237030600002
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/93659]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Dalian Fisheries Univ, Key & Open Lab Aquat Prod Proc & Utilizat Liaonin, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Xiong, CN,Li, W,Liu, H,et al. A normal mucin-binding lectin from the sponge Craniella australiensis[J]. comparative biochemistry and physiology c-toxicology & pharmacology,2006,143(1):9-16.
APA	Xiong, CN.,Li, W.,Liu, H.,Zhang, W.,Dou, JL.,...&Ma, XJ.(2006).A normal mucin-binding lectin from the sponge Craniella australiensis.comparative biochemistry and physiology c-toxicology & pharmacology,143(1),9-16.
MLA	Xiong, CN,et al."A normal mucin-binding lectin from the sponge Craniella australiensis".comparative biochemistry and physiology c-toxicology & pharmacology 143.1(2006):9-16.