Site-Specific N-Glycan Characterization of Grass Carp Serum IgM

doi:10.3389/fimmu.2018.02645

CORC > 武汉植物园 > 中国科学院武汉植物园

	Site-Specific N-Glycan Characterization of Grass Carp Serum IgM
	Geng, Hui 6; Zhang, Yong-An 1,2,5; Xiong, Li 6; Wan, Cui-Hong 6; Cui, Yan-Fang 3; Yang, Qian 6; Bai, Hao 6; Wan, Jian 6; Cui, Zheng-Wei 4,5; Hu, Meng-Die 6
刊名	FRONTIERS IN IMMUNOLOGY
	2018-11-14
卷号	9 页码:16
关键词	teleost grass carp imrnunoglobulin M N-glycan liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) glycosylation matrix assisted laser desorption/ionization-time-offlight-MS (MALDI-TOF-MS)
ISSN号	1664-3224
DOI	10.3389/fimmu.2018.02645
英文摘要	Immunoglobulin M (IgM) is the major antibody in teleost fish and plays an important role in humoral adaptive immunity. The N-linked carbohydrates presenting on IgM have been well documented in higher vertebrates, but little is known regarding site-specific N-glycan characteristics in teleost IgM. In order to characterize these site-specific N-glycans, we conducted the first study of the N-glycans of each glycosylation site of the grass carp serum IgM. Among the four glycosylation sites, the Asn-262, Asn-303, and Asn-426 residues were efficiently glycosylated, while Asn-565 at the C-terminal tailpiece was incompletely occupied. A striking decrease in the level of occupancy at the Asn-565 glycosite was observed in dimeric IgM compared to that in monomeric IgM, and no glycan occupancy of Asn-565 was observed in tetrameric IgM. Glycopeptide analysis with liquid chromatography-electrospray ionization tandem mass spectrometry revealed mainly complex-type glycans with substantial heterogeneity, with neutral; monosialyl-, disialyl- and trisialylated; and fucosyl-and non-fucosyl-oligosaccharides conjugated to grass carp serum IgM. Glycan variation at a single site was greatest at the Asn-262 glycosite. Unlike IgMs in other species, only traces of complex-type and no high-mannose glycans were found at the Asn-565 glycosite. Matrix-assisted laser desorption ionization analysis of released glycans confirmed the overwhelming majority of carbohydrates were of the complex-type. These results indicate that grass carp serum IgM exhibits unique N-glycan features and highly processed oligosaccharides attached to individual glycosites.
资助项目	Natural Science Foundation of China[21473065] ; Fundamental Research Funds for the Central University of Central China Normal University[CCNU16A02044] ; Hubei Province Natural Science Key Foundation Project[2014CFA079] ; National Basic Research Program of China[2014CB138601]
WOS研究方向	Immunology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000450107300001
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/6114]
专题	中国科学院武汉植物园
通讯作者	Geng, Hui; Zhang, Yong-An
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 2.Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China 3.Cent China Normal Univ, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan, Hubei, Peoples R China 4.Univ Chinese Acad Sci, Coll Modem Agr Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China 6.Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Geng, Hui,Zhang, Yong-An,Xiong, Li,et al. Site-Specific N-Glycan Characterization of Grass Carp Serum IgM[J]. FRONTIERS IN IMMUNOLOGY,2018,9:16.
APA	Geng, Hui.,Zhang, Yong-An.,Xiong, Li.,Wan, Cui-Hong.,Cui, Yan-Fang.,...&Su, Yi-Ling.(2018).Site-Specific N-Glycan Characterization of Grass Carp Serum IgM.FRONTIERS IN IMMUNOLOGY,9,16.
MLA	Geng, Hui,et al."Site-Specific N-Glycan Characterization of Grass Carp Serum IgM".FRONTIERS IN IMMUNOLOGY 9(2018):16.