Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter

doi:10.7717/peerj.5701

CORC > 武汉植物园 > 中国科学院武汉植物园

	Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter
	Long, Yong 4; Yang, Guoliang 1; Cui, Zongbin 4; Gao, Qiang 2; Yan, Junjun 3,4
刊名	PEERJ
	2018-10-23
卷号	6 页码:18
关键词	Giant freshwater prawn Beta-actin gene Promoter Transcriptional activity
ISSN号	2167-8359
DOI	10.7717/peerj.5701
英文摘要	Constitutive promoters are important tools for gene function studies and transgenesis. The Beta-actin (actb1) gene promoter has been isolated from many species but remains to be cloned from the giant freshwater prawn (Macrobrachium rosenbergii). In this study, we cloned and characterized the Mractb1 gene promoter. Two alternative promoters were identified for the Mractb1 gene, which direct the generation of two transcripts with different 5' untranslated regions. Three CpG islands were predicted in the upstream sequence, which are intimately related to transcription initiation and promoter activity. In addition to the CCAAT-box and the CArG-box, molecular dissection of the flanking sequence revealed the existence of one negative and two positive elements in the upstream region and the first intron. Finally, the Mractb1 promoter demonstrated comparative activity to the carp (Cyprinus carpio) actb1 promoter. Our investigations provide a valuable genetic tool for gene function studies and shed light on the regulation of the Mractb1 gene.
资助项目	Youth Innovation Promotion Association of Chinese Academy of Sciences[2016305] ; Major Project for New Agricultural Varieties Breeding of Zhejiang Province[2012C12907-2]
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	PEERJ INC
WOS记录号	WOS:000448082800004
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/5852]
专题	中国科学院武汉植物园
通讯作者	Long, Yong; Yang, Guoliang
作者单位	1.Huzhou Univ, Huzhou, Peoples R China 2.Zhejiang Inst Freshwater Fisheries, Huzhou, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Long, Yong,Yang, Guoliang,Cui, Zongbin,et al. Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter[J]. PEERJ,2018,6:18.
APA	Long, Yong,Yang, Guoliang,Cui, Zongbin,Gao, Qiang,&Yan, Junjun.(2018).Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter.PEERJ,6,18.
MLA	Long, Yong,et al."Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii) beta-actin gene promoter".PEERJ 6(2018):18.