Whole Genome Incorporation and Epigenetic Stability in a Newly Synthetic Allopolyploid of Gynogenetic Gibel Carp

doi:10.1093/gbe/evy165

CORC > 武汉植物园 > 中国科学院武汉植物园

	Whole Genome Incorporation and Epigenetic Stability in a Newly Synthetic Allopolyploid of Gynogenetic Gibel Carp
	Gui, Jian-Fang 1,2; Zhou, Li 1,2; Zhang, Xiao-Juan 2; Li, Zhi 2; Wang, Zhong-Wei 1,2; Wang, Yang 1,2; Li, Xi-Yin 1,2; Shao, Guang-Ming 1,2
刊名	GENOME BIOLOGY AND EVOLUTION
	2018-09-01
卷号	10 期号:9 页码:2394-2407
关键词	hybridization synthetic allopolyploid gibel carp gynogenesis epigenetics cytosine methylation pattern
ISSN号	1759-6653
DOI	10.1093/gbe/evy165
英文摘要	Allopolyploidization plays an important role in speciation, and some natural or synthetic allopolyploid fishes have been extensively applied to aquaculture. Although genetic and epigenetic inheritance and variation associated with plant allopolyploids have been well documented, the relative research in allopolyploid animals is scarce. In this study, the genome constitution and DNA methylation inheritance in a newly synthetic allopolyploid of gynogenetic gibel carp were analyzed. The incorporation of a whole genome of paternal common carp sperm in the allopolyploid was confirmed by genomic in situ hybridization, chromosome localization of 45S rDNAs, and sequence comparison. Pooled sample-based methylation sensitive amplified polymorphism (MSAP) revealed that an overwhelming majority (98.82%) of cytosine methylation patterns in the allopolyploid were inherited from its parents of hexaploid gibel carp clone D and common carp. Compared to its parents, 11 DNA fragments in the allopolyploid were proved to be caused by interindividual variation, recombination, deletion, and mutation through individual sample-based MSAP and sequencing. Contrast to the rapid and remarkable epigenetic changes in most of analyzed neopolyploids, no cytosine methylation variation was detected in the gynogenetic allopolyploid. Therefore, the newly synthetic allopolyploid of gynogenetic gibel carp combined genomes from its parents and maintained genetic and epigenetic stability after its formation and subsequently seven successive gynogenetic generations. Our current results provide a paradigm for recurrent polyploidy consequences in the gynogenetic allopolyploid animals.
资助项目	Key Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDY-SSW-SMC025] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA08030201] ; earmarked fund for Modern Agro-industry Technology Research System[NYCYTX-49] ; Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ01]
WOS研究方向	Evolutionary Biology ; Genetics & Heredity
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000446102700019
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/5834]
专题	中国科学院武汉植物园
通讯作者	Gui, Jian-Fang; Zhou, Li
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Gui, Jian-Fang,Zhou, Li,Zhang, Xiao-Juan,et al. Whole Genome Incorporation and Epigenetic Stability in a Newly Synthetic Allopolyploid of Gynogenetic Gibel Carp[J]. GENOME BIOLOGY AND EVOLUTION,2018,10(9):2394-2407.
APA	Gui, Jian-Fang.,Zhou, Li.,Zhang, Xiao-Juan.,Li, Zhi.,Wang, Zhong-Wei.,...&Shao, Guang-Ming.(2018).Whole Genome Incorporation and Epigenetic Stability in a Newly Synthetic Allopolyploid of Gynogenetic Gibel Carp.GENOME BIOLOGY AND EVOLUTION,10(9),2394-2407.
MLA	Gui, Jian-Fang,et al."Whole Genome Incorporation and Epigenetic Stability in a Newly Synthetic Allopolyploid of Gynogenetic Gibel Carp".GENOME BIOLOGY AND EVOLUTION 10.9(2018):2394-2407.