Characterization of Microcystis (Cyanobacteria) Genotypes Based on the Internal Transcribed Spacer Region of rRNA by Next-Generation Sequencing

doi:10.3389/fmicb.2018.00971

CORC > 武汉植物园 > 中国科学院武汉植物园

	Characterization of Microcystis (Cyanobacteria) Genotypes Based on the Internal Transcribed Spacer Region of rRNA by Next-Generation Sequencing
	Liu, Xiang 1; Yu, Gongliang 1; Li, Renhui 1; Huo, Da 1,2; Qiao, Zhiyi 2; Chen, Youxin 1; Zheng, Tao 2; Zhang, Xinyue 2
刊名	FRONTIERS IN MICROBIOLOGY
	2018-05-15
卷号	9 页码:12
关键词	cyanobacteria Yuqiao reservoir Haihe River high throughput sequencing ITS region
ISSN号	1664-302X
DOI	10.3389/fmicb.2018.00971
英文摘要	Microcystis is one of the most common and dominant bloom-forming cyanobacteria in freshwater worldwide. The method for genotype detection based on traditional molecular cloning is expensive and time consuming and generates a limited number of sequences. In this study, a high-throughput sequencing (HTS) method was developed to detect the internal transcribed spacer (ITS) regions between 16S and 23S rRNA region of Microcystis populations along a typical water system in Yuqiao Reservoir-Haihe River in Tianjin, northern China. A total of 629,341 reads were obtained and clustered into 2005 operational taxonomic units (OTUs). Analysis of alpha diversity indices showed that the Haihe River is more diverse than Yuqiao Reservoir. In general, the two water areas exhibit a clear differentiation pattern in OTU abundance, sharing genotypes from a small part of Yuqiao Reservoir with those in the Haihe River. Phylogenetic analysis further indicated the possible flexible evolution of Microcystis genotypes occurring in the research areas. This study provides the first exhaustive description of HTS method for detection of ITS region to evaluate Microcystis intra-species diversity and relationship.
资助项目	National Science Foundation of China[NSFC51779247] ; International Science and Technology Cooperation Program of China[2013DFA71340] ; Tianjin Agricultural University Key Laboratory of Aqua-ecology platform project
WOS研究方向	Microbiology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000432290000001
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/5303]
专题	中国科学院武汉植物园
通讯作者	Li, Renhui; Qiao, Zhiyi
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China 2.Tianjin Agr Univ, Dept Fisheries Sci, Natl Demonstrat Ctr Expt Aquaecol & Aquaculture E, Tianjin, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xiang,Yu, Gongliang,Li, Renhui,et al. Characterization of Microcystis (Cyanobacteria) Genotypes Based on the Internal Transcribed Spacer Region of rRNA by Next-Generation Sequencing[J]. FRONTIERS IN MICROBIOLOGY,2018,9:12.
APA	Liu, Xiang.,Yu, Gongliang.,Li, Renhui.,Huo, Da.,Qiao, Zhiyi.,...&Zhang, Xinyue.(2018).Characterization of Microcystis (Cyanobacteria) Genotypes Based on the Internal Transcribed Spacer Region of rRNA by Next-Generation Sequencing.FRONTIERS IN MICROBIOLOGY,9,12.
MLA	Liu, Xiang,et al."Characterization of Microcystis (Cyanobacteria) Genotypes Based on the Internal Transcribed Spacer Region of rRNA by Next-Generation Sequencing".FRONTIERS IN MICROBIOLOGY 9(2018):12.