Investigation of benzylisoquinoline alkaloid biosynthetic pathway and its transcriptional regulation in lotus

doi:10.1038/s41438-018-0035-0

CORC > 武汉植物园 > 中国科学院武汉植物园

	Investigation of benzylisoquinoline alkaloid biosynthetic pathway and its transcriptional regulation in lotus
	Ogutu, Collins 1,2; Yang, Dong 1; Liu, Yanling 1; Vimolmangkang, Sornkanok 4; Han, Yuepeng 1,3; Deng, Xianbao 1; Zhao, Li 1,2; Fang, Ting 1,2; Xiong, Yaqian 1
刊名	HORTICULTURE RESEARCH
	2018-06-01
卷号	5 页码:16
ISSN号	2052-7276
DOI	10.1038/s41438-018-0035-0
英文摘要	Lotus predominantly accumulates benzylisoquinoline alkaloids (BIAs), but their biosynthesis and regulation remain unclear. Here, we investigated structural and regulatory genes involved in BIA accumulation in lotus. Two clustered CYP80 genes were identified to be responsible for the biosynthesis of bis-BIAS and aporphine-type BIAs, respectively, and their tissue-specific expression causes divergence in alkaloid component between leaf and embryo. In contrast with the common (S)-reticuline precursor for most BIAs, aporphine alkaloids in lotus leaf may result from the (S)-Nmethylcoclaurine precursor. Structural diversity of BIA alkaloids in the leaf is attributed to enzymatic modifications, including intramolecular C-C phenol coupling on ring A and methylation and demethylation at certain positions. Additionally, most BIA biosynthetic pathway genes show higher levels of expression in the leaf of high-BIA cultivar compared with low-BIA cultivar, suggesting transcriptional regulation of BIA accumulation in lotus. Five transcription factors, including three MYBs, one ethylene-responsive factor, and one basic helix-loop-helix (bHLH), were identified to be candidate regulators of BIA biosynthesis in lotus. Our study reveals a BIA biosynthetic pathway and its transcriptional regulation in lotus, which will enable a deeper understanding of BIA biosynthesis in plants.
资助项目	National Natural Science Foundation of China[31450110420] ; National Natural Science Foundation of China[31272195] ; Overseas Construction Plan for Science and Education Base, China-Africa Center for Research and Education, Chinese Academy of Sciences[SAJC201327]
WOS研究方向	Plant Sciences ; Agriculture
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000433920100003
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/5278]
专题	中国科学院武汉植物园
通讯作者	Han, Yuepeng
作者单位	1.Chinese Acad Sci, Key Lab Plant Germplasrn Enhancement & Specialty, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 2.Grad Univ Chinese Acad Sci, 19A Yuquanlu, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Sinoafrican Joint Res Ctr, Wuhan 430074, Hubei, Peoples R China 4.Chulalongkorn Univ, Fac Pharmaceut Sci, Dept Biochem & Microbiol, Bangkok 10330, Thailand
推荐引用方式 GB/T 7714	Ogutu, Collins,Yang, Dong,Liu, Yanling,et al. Investigation of benzylisoquinoline alkaloid biosynthetic pathway and its transcriptional regulation in lotus[J]. HORTICULTURE RESEARCH,2018,5:16.
APA	Ogutu, Collins.,Yang, Dong.,Liu, Yanling.,Vimolmangkang, Sornkanok.,Han, Yuepeng.,...&Xiong, Yaqian.(2018).Investigation of benzylisoquinoline alkaloid biosynthetic pathway and its transcriptional regulation in lotus.HORTICULTURE RESEARCH,5,16.
MLA	Ogutu, Collins,et al."Investigation of benzylisoquinoline alkaloid biosynthetic pathway and its transcriptional regulation in lotus".HORTICULTURE RESEARCH 5(2018):16.