Dietary arginine supplementation mitigates the soybean meal induced enteropathy in juvenile turbot, Scophthalmus maximus L.

doi:10.1111/are.13608

CORC > 武汉植物园 > 中国科学院武汉植物园

	Dietary arginine supplementation mitigates the soybean meal induced enteropathy in juvenile turbot, Scophthalmus maximus L.
	Hu, Haibin 1; Chen, Zhichu 1; Liu, Yang 1; Li, Yanxian 1; Yang, Pei 1; Yu, Guijuan 1; Ai, Qinghui 1; Xu, Wei 1; Zhang, Wenbing 1; Zhang, Yongan 2
刊名	AQUACULTURE RESEARCH
	2018-04-01
卷号	49 期号:4 页码:1535-1545
关键词	arginine enteropathy intestinal mucosal barrier soybean meal turbot
ISSN号	1355-557X
DOI	10.1111/are.13608
英文摘要	A 12-week feeding trial was conducted to investigate the protective effects of arginine on the intestinal health of juvenile turbot (Scophthalmus maximus L.) fed high doses of dietary soybean meal. Sextuple replicates of 30 fish were fed each of four isonitrogenous and isolipidic experimental diets: fish-meal-based diet (FM); FM with 40% fish meal protein replaced by soybean meal protein (SBM); SBM+1% arginine (ARG1) and SBM+2% arginine (ARG2). Turbot-fed SBM showed typical soybean meal-induced enteropathy, characterized by an increase in the thickness of lamina propria, as well as significant decreases in the absorptive surface and activities of intestinal brush border membrane enzymes and Na+, K+-ATPase. On the contrary, fish-fed ARG1 showed enhanced intestinal mucosal barrier function in terms of the enhanced gene expression of anti-inflammatory cytokine and barrier-forming tight junction proteins, as well as depressed gene expression of pro-inflammatory cytokines and pore-forming tight junction proteins. Fish-fed AGR2 showed intermediate intestinal performances between SBM and AGR1. Dietary arginine (1%) also significantly regulated the expression of AMP-activated protein kinase alpha 1 (AMPK alpha 1), myosin light chain kinase (MLCK) and nuclear transcription factor-kappa B p65 (NF-kappa B p65), and these regulations correlated well with its regulations on intestinal mucosal barrier related genes at all sampling time-points. In conclusion, arginine supplementation (1%) in diet for turbot mitigated the soybean meal-induced enteropathy by enhancing the intestinal mucosal barrier function. The activation of AMPK alpha 1 signalling molecule as well as the suppression of NF-kappa B p65 and MLCK signalling molecules may mediate the beneficial effects of arginine.
资助项目	National Basic Research Program[2014CB138601] ; National Natural Science Foundation of China[41576137] ; National Natural Science Foundation of China[31202006] ; China Agriculture Researches System[CARS 50-G08]
WOS研究方向	Fisheries
语种	英语
出版者	WILEY
WOS记录号	WOS:000427003200017
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/5160]
专题	中国科学院武汉植物园
通讯作者	Zhang, Yanjiao
作者单位	1.Ocean Univ China, Key Lab Mariculture, Minist Educ, Key Lab Aquaculture Nutr & Feed,Minist Agr, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Hu, Haibin,Chen, Zhichu,Liu, Yang,et al. Dietary arginine supplementation mitigates the soybean meal induced enteropathy in juvenile turbot, Scophthalmus maximus L.[J]. AQUACULTURE RESEARCH,2018,49(4):1535-1545.
APA	Hu, Haibin.,Chen, Zhichu.,Liu, Yang.,Li, Yanxian.,Yang, Pei.,...&Mai, Kangsen.(2018).Dietary arginine supplementation mitigates the soybean meal induced enteropathy in juvenile turbot, Scophthalmus maximus L..AQUACULTURE RESEARCH,49(4),1535-1545.
MLA	Hu, Haibin,et al."Dietary arginine supplementation mitigates the soybean meal induced enteropathy in juvenile turbot, Scophthalmus maximus L.".AQUACULTURE RESEARCH 49.4(2018):1535-1545.