Functional characterization of a short peptidoglycan recognition protein from Chinese giant salamander (Andrias davidianus)

doi:10.18632/oncotarget.21470

CORC > 武汉植物园 > 中国科学院武汉植物园

	Functional characterization of a short peptidoglycan recognition protein from Chinese giant salamander (Andrias davidianus)
	Qi, Zhitao 1; Ren, Shisi 2; Zhang, Qihuan 1; Zou, Jun 3; Xu, Qiaoqing 4; Wang, Zisheng 1; Qiao, Guo 1; Nie, Pin 2,5; Chang, Mingxian 2,5
刊名	ONCOTARGET
	2017-11-21
卷号	8 期号:59 页码:99323-99335
关键词	peptidoglycan recognition protein andrias davidianus gene clone functional analysis Immunology and Microbiology Section Immune response Immunity
ISSN号	1949-2553
DOI	10.18632/oncotarget.21470
英文摘要	Peptidoglycan (PGN) recognition proteins (PGRPs) are important pattern recognition receptors (PRRs) involved in immune defense against bacterial infections. In this study, a short PGRP (termed AdPGRP-S1) was cloned and functionally characterized from Chinese giant salamander (Andrias davidianus), the largest extant urodela amphibian species. AdPGRP-S1 was 184 aa in length and shared 38.7%-54.9% sequence identities with other vertebrates' short PGRPs. It contained one typical PGRP domain at the C-terminal region and several conserved amino acid (aa) residues involved in amidase and PGN binding. AdPGRP-S1 was constitutively expressed in all tissues examined, with the highest expression level seen in spleen and intestine. It has been shown that AdPGRP-S1 could bind and degrade Lys-PGN and Dap-PGN. Further, AdPGRP-S1 had antibacterial activity against the Gram-negative bacteria, Edwardsiella tarda, and was able to trigger the activation of NF-kappa B signaling. These results demonstrated that AdPGRP-S1 possesses multiple functions in pathogen recognition, mediating ceullular signaling, and initiating antibacterial response. This is the first functional study of a salamander PGRP, providing insight to further understand the functional evolution of verterbates' PGRPs.
资助项目	National Natural Science Foundation of China[31302221] ; National Natural Science Foundation of China[31172408] ; National Natural Science Foundation of China[31272666] ; Jiangsu Province[BK20171274] ; Jiangsu Province[BK2011418] ; Opening Project of Jiangsu Key Laboratory of Biochemistry and Biotechnology of Marine Wetland[K2016-08] ; Qinglan project of Jiangsu province of China
WOS研究方向	Oncology ; Cell Biology
语种	英语
出版者	IMPACT JOURNALS LLC
WOS记录号	WOS:000419561600019
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/4633]
专题	中国科学院武汉植物园
通讯作者	Qi, Zhitao; Chang, Mingxian
作者单位	1.Yancheng Inst Technol, Jiangsu Key Lab Biochem & Biotechnol Marine Wetla, Yancheng, Jiangsu, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China 3.Univ Aberdeen, Scottish Fish Immunol Res Ctr, Aberdeen, Scotland 4.Yangtze Univ, Coll Anim Sci, Jingzhou, Hubei, Peoples R China 5.Minist Agr, Key Lab Aquaculture Dis Control, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Qi, Zhitao,Ren, Shisi,Zhang, Qihuan,et al. Functional characterization of a short peptidoglycan recognition protein from Chinese giant salamander (Andrias davidianus)[J]. ONCOTARGET,2017,8(59):99323-99335.
APA	Qi, Zhitao.,Ren, Shisi.,Zhang, Qihuan.,Zou, Jun.,Xu, Qiaoqing.,...&Chang, Mingxian.(2017).Functional characterization of a short peptidoglycan recognition protein from Chinese giant salamander (Andrias davidianus).ONCOTARGET,8(59),99323-99335.
MLA	Qi, Zhitao,et al."Functional characterization of a short peptidoglycan recognition protein from Chinese giant salamander (Andrias davidianus)".ONCOTARGET 8.59(2017):99323-99335.