Comprehensive Transcriptome Profiling and Identification of Potential Genes Responsible for Salt Tolerance in Tall Fescue Leaves under Salinity Stress

doi:10.3390/genes9100466

CORC > 武汉植物园 > 中国科学院武汉植物园

	Comprehensive Transcriptome Profiling and Identification of Potential Genes Responsible for Salt Tolerance in Tall Fescue Leaves under Salinity Stress
	Amombo, Erick 1,2,4; Li, Xiaoning 1,2,4; Wang, Guangyang 1,2,4; An, Shao 3; Wang, Wei 3; Fu, Jinmin 3
刊名	GENES
	2018-10-01
卷号	9 期号:10 页码:20
关键词	tall fescue salinity stress photosynthesis RNA-sequencing simple sequence repeats transcription factors
ISSN号	2073-4425
DOI	10.3390/genes9100466
英文摘要	Soil salinity is a serious threat to plant growth and crop productivity. Tall fescue utilization in saline areas is limited by its inferior salt tolerance. Thus, a transcriptome study is a prerequisite for future research aimed at providing deeper insights into the molecular mechanisms of tall fescue salt tolerance as well as molecular breeding. Recent advances in sequencing technology offer a platform to achieve this. Here, Illumina RNA sequencing of tall fescue leaves generated a total of 144,339 raw reads. After de novo assembly, unigenes with a total length of 129,749,938 base pairs were obtained. For functional annotations, the unigenes were aligned to various databases. Further structural analyses revealed 79,352 coding DNA sequences and 13,003 microsatellites distributed across 11,277 unigenes as well as single nucleotide polymorphisms. In total, 1862 unigenes were predicted to encode for 2120 transcription factors among which most were key salt-responsive. We determined differential gene expression and distribution per sample and most genes related to salt tolerance and photosynthesis were upregulated in 48 h vs. 24 h salt treatment. Protein interaction analysis revealed a high interaction of chaperonins and Rubisco proteins in 48 h vs. 24 h salt treatment. The gene expressions were finally validated using quantitative polymerase chain reaction (qPCR), which was coherent with sequencing results.
资助项目	China National Science Foundation (NSFC)[31470363] ; China National Science Foundation (NSFC)[31772662]
WOS研究方向	Genetics & Heredity
语种	英语
出版者	MDPI
WOS记录号	WOS:000448656700004
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/3440]
专题	中国科学院武汉植物园
通讯作者	Fu, Jinmin
作者单位	1.Chinese Acad Sci Wuhan, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China 2.Univ Chinese Acad Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China 3.Ludong Univ, Inst Adv Studies Coastal Ecol, Yantai 264000, Peoples R China 4.Chinese Acad Sci Wuhan, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Amombo, Erick,Li, Xiaoning,Wang, Guangyang,et al. Comprehensive Transcriptome Profiling and Identification of Potential Genes Responsible for Salt Tolerance in Tall Fescue Leaves under Salinity Stress[J]. GENES,2018,9(10):20.
APA	Amombo, Erick,Li, Xiaoning,Wang, Guangyang,An, Shao,Wang, Wei,&Fu, Jinmin.(2018).Comprehensive Transcriptome Profiling and Identification of Potential Genes Responsible for Salt Tolerance in Tall Fescue Leaves under Salinity Stress.GENES,9(10),20.
MLA	Amombo, Erick,et al."Comprehensive Transcriptome Profiling and Identification of Potential Genes Responsible for Salt Tolerance in Tall Fescue Leaves under Salinity Stress".GENES 9.10(2018):20.