The GARP/MYB-related grape transcription factor AQUILO improves cold tolerance and promotes the accumulation of raffinose family oligosaccharides

doi:10.1093/jxb/ery020

CORC > 武汉植物园 > 中国科学院武汉植物园

	The GARP/MYB-related grape transcription factor AQUILO improves cold tolerance and promotes the accumulation of raffinose family oligosaccharides
	Sun, Xiaoming 1,2; Tomas Matus, Jose 3; Wong, Darren Chern Jan 4; Wang, Zemin 1; Chai, Fengmei 2; Zhang, Langlang 2; Fang, Ting 2; Zhao, Li 2; Wang, Yi 1; Han, Yuepeng 2
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2018-03-16
卷号	69 期号:7 页码:1749-1764
关键词	Abiotic stress Chinese wild grape cold response galactinol synthase grape transformation HRS1 low temperature exotherms raffinose ROS
ISSN号	0022-0957
DOI	10.1093/jxb/ery020
英文摘要	Grapevine (Vitis vinifera L.) is a widely cultivated fruit crop whose growth and productivity are greatly affected by low temperatures. On the other hand, wild Vitis species represent valuable genetic resources of natural stress tolerance. We have isolated and characterized a MYB-like gene encoding a putative GARP-type transcription factor from Amur grape (V. amurensis) designated as VaAQUILO. AQUILO (AQ) is induced by cold in both V. amurensis and V. vinifera, and its overexpression results in significantly improved tolerance to cold both in transgenic Arabidopsis and in Amur grape calli. In Arabidopsis, the ectopic expression of VaAQ increased antioxidant enzyme activities and up-regulated reactive oxygen species-(ROS) scavenging-related genes. Comparative mRNA sequencing profiling of 35S: VaAQ Arabidopsis plants suggests that this transcription factor is related to phosphate homeostasis like their Arabidopsis closest homologues: AtHRS1 and AtHHO2. However, when a cold stress is imposed, AQ is tightly associated with the cold-responsive pathway and with the raffinose family oligosaccharides (RFOs), as observed by the up-regulation of galactinol synthase (GoLS) and raffinose synthase genes. Gene co-expression network (GCN) and cis-regulatory element (CRE) analyses in grapevine indicated AQ as potentially regulating VvGoLS genes. Increased RFO content was confirmed in both transgenic Arabidopsis and Amur grape calli overexpressing VaAQ. Taken together, our results imply that AQ improves cold tolerance through promoting the accumulation of osmoprotectants.
资助项目	Youth Innovation Promotion Association of CAS[2015281] ; China Postdoctoral Science Foundation[2016M601166] ; Science and Technology Service Network Initiative of CAS[KFJ-STS-ZDTP-025] ; Grape Breeding Project of Ningxia[NXNYYZ201502]
WOS研究方向	Plant Sciences
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000428655800023
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/3389]
专题	中国科学院武汉植物园
通讯作者	Liang, Zhenchang; Xin, Haiping
作者单位	1.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China 3.CSIC IRTA UAB UB, CRAG, Barcelona 08193, Spain 4.Australian Natl Univ, Res Sch Biol, Ecol & Evolut, Acton, ACT 2601, Australia
推荐引用方式 GB/T 7714	Sun, Xiaoming,Tomas Matus, Jose,Wong, Darren Chern Jan,et al. The GARP/MYB-related grape transcription factor AQUILO improves cold tolerance and promotes the accumulation of raffinose family oligosaccharides[J]. JOURNAL OF EXPERIMENTAL BOTANY,2018,69(7):1749-1764.
APA	Sun, Xiaoming.,Tomas Matus, Jose.,Wong, Darren Chern Jan.,Wang, Zemin.,Chai, Fengmei.,...&Xin, Haiping.(2018).The GARP/MYB-related grape transcription factor AQUILO improves cold tolerance and promotes the accumulation of raffinose family oligosaccharides.JOURNAL OF EXPERIMENTAL BOTANY,69(7),1749-1764.
MLA	Sun, Xiaoming,et al."The GARP/MYB-related grape transcription factor AQUILO improves cold tolerance and promotes the accumulation of raffinose family oligosaccharides".JOURNAL OF EXPERIMENTAL BOTANY 69.7(2018):1749-1764.