Construction of a SNP-based genetic linkage map for kiwifruit using next-generation restriction-site-associated DNA sequencing (RADseq)

doi:10.1007/s11032-017-0729-2

CORC > 武汉植物园 > 中国科学院武汉植物园

	Construction of a SNP-based genetic linkage map for kiwifruit using next-generation restriction-site-associated DNA sequencing (RADseq)
	Liu, Chun-Yan 1; Li, Da-Wei 1; Zhou, Ju-Hong 2; Zhang, Qiong 1; Tian, Hua 1; Yao, Xiao-Hong 1
刊名	MOLECULAR BREEDING
	2017-11-01
卷号	37 期号:11 页码:1-7
关键词	Kiwifruit Genetic mapping Molecular marker RADseq
ISSN号	1380-3743
DOI	10.1007/s11032-017-0729-2
英文摘要	Kiwifruit is a perennial horticultural crop species of the Actinidiaceae family and has high nutritional value. For a species with a long generation time, traditional breeding and genetic improvement is predicted to take more than 20 years to obtain superior cultivars. Thus, marker-assisted selection (MAS) should be used to accelerate the breeding process. Development of a genetic linkage map and molecular markers are pre-requisites for MAS of crop species. Here, we report a genome-wide SNP-based genetic map of kiwifruit by analysing next-generation restriction-site-associated DNA sequencing (RADseq) reads. To construct a genetic linkage map, a 102 F1 line mapping population of Actinidia chinensis (2n = 58) was derived by combining parents that had contrasting phenotypic traits. The maternal map contained 4112 SNP loci and spanned a distance of 3821 cM, with an average adjacent-marker interval length of 0.929 cM. The map length of the 29 linkage groups ranged from 78.3 to 169.9 cM, with an average length of 131.8 cM. High levels of collinearity between the 29 genetic maps with the kiwifruit reference genome were found. The genetic map developed in this study can serve as an important platform to improve kiwifruit research, including anchoring unmapped scaffolds of the kiwifruit genome sequence and mapping QTLs (quantitative trait loci) that control economically important traits.
资助项目	Natural Scientific Foundation of China[31770374] ; Backbone Talent Program of the Wuhan Botanical Garden, Chinese Academy of Sciences[Y655291A04]
WOS研究方向	Agriculture ; Plant Sciences ; Genetics & Heredity
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000414010900001
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/1292]
专题	中国科学院武汉植物园
通讯作者	Yao, Xiao-Hong
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan 430074, Hubei, Peoples R China 2.Beijing Genom Inst BGI Shenzhen, Shenzhen 518083, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Liu, Chun-Yan,Li, Da-Wei,Zhou, Ju-Hong,et al. Construction of a SNP-based genetic linkage map for kiwifruit using next-generation restriction-site-associated DNA sequencing (RADseq)[J]. MOLECULAR BREEDING,2017,37(11):1-7.
APA	Liu, Chun-Yan,Li, Da-Wei,Zhou, Ju-Hong,Zhang, Qiong,Tian, Hua,&Yao, Xiao-Hong.(2017).Construction of a SNP-based genetic linkage map for kiwifruit using next-generation restriction-site-associated DNA sequencing (RADseq).MOLECULAR BREEDING,37(11),1-7.
MLA	Liu, Chun-Yan,et al."Construction of a SNP-based genetic linkage map for kiwifruit using next-generation restriction-site-associated DNA sequencing (RADseq)".MOLECULAR BREEDING 37.11(2017):1-7.