Microcystin-RR induced apoptosis in tobacco BY-2 suspension cells is mediated by reactive oxygen species and mitochondrial permeability transition pore status

doi:10.1016/j.tiv.2007.09.018

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	Microcystin-RR induced apoptosis in tobacco BY-2 suspension cells is mediated by reactive oxygen species and mitochondrial permeability transition pore status
	Li, Dunhai 2; Huang, Wenmin 1,2; Xing, Wei 3; Liu, Yongding 2
刊名	TOXICOLOGY IN VITRO
	2008-03-01
卷号	22 期号:2 页码:328-337
关键词	MC-RR tobacco BY-2 suspension cells apoptosis ROS PTP
ISSN号	0887-2333
DOI	10.1016/j.tiv.2007.09.018
英文摘要	When tobacco BY-2 cells were treated with 60 mu g/mL MC-RR for 5 d, time-dependent effects of MC-RR on the cells were observed. Morphological changes such as abnormal elongation, evident chromatin condensation and margination, fragmentation of nucleus and formation of apoptotic-like bodies suggest that 60 mu g/mL MC-RR induced rapid apoptosis in tobacco BY-2 cells. Moreover, there was a significant and rapid increase of ROS level before the loss of mitochondrial membrane potential (Delta Psi(m)) and the onset of cell apoptosis. Ascorbic acid (AsA), a major primary antioxidant, prevented the increase of ROS generation, blocked the decrease in Delta Psi(m) and subsequent cell apoptosis, indicating a critical role of ROS in serving as an important signaling molecule by causing a reduction of Delta Psi(m) and MC-RR-induced tobacco BY-2 cell apoptosis. In addition, a specific mitochondrial permeability transition pores (PTP) inhibitor, cyclosporin A (CsA), significantly blocked the MC-RR-induced ROS formation, loss of Delta Psi(m), as well as cell apoptosis when the cells were MC-RR stressed for 3 d, suggesting that PTP is involved in 60 mu g/mL MC-RR-induced tobacco cell apoptosis signalling process. Thus, we concluded that the mechanism of MC-RR-induced apoptosis signalling pathways in tobacco BY-2 cells involves not only the excess generation of ROS and oxidative stress, but also the opening of PTP inducing loss of mitochondrial membrane potential. (C) 2007 Published by Elsevier Ltd.
WOS研究方向	Toxicology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000254694500007
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/1109]
专题	中国科学院武汉植物园
通讯作者	Liu, Yongding
作者单位	1.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 3.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Li, Dunhai,Huang, Wenmin,Xing, Wei,et al. Microcystin-RR induced apoptosis in tobacco BY-2 suspension cells is mediated by reactive oxygen species and mitochondrial permeability transition pore status[J]. TOXICOLOGY IN VITRO,2008,22(2):328-337.
APA	Li, Dunhai,Huang, Wenmin,Xing, Wei,&Liu, Yongding.(2008).Microcystin-RR induced apoptosis in tobacco BY-2 suspension cells is mediated by reactive oxygen species and mitochondrial permeability transition pore status.TOXICOLOGY IN VITRO,22(2),328-337.
MLA	Li, Dunhai,et al."Microcystin-RR induced apoptosis in tobacco BY-2 suspension cells is mediated by reactive oxygen species and mitochondrial permeability transition pore status".TOXICOLOGY IN VITRO 22.2(2008):328-337.