CHARACTERIZATION OF 15 NOVEL SINGLE NUCLEOTIDE POLYMORPHISMS (SNPS) IN THE ACTINIDIA CHINENSIS SPECIES COMPLEX (ACTINIDIACEAE)

doi:10.3732/ajb.1000442

CORC > 武汉植物园 > 中国科学院武汉植物园

	CHARACTERIZATION OF 15 NOVEL SINGLE NUCLEOTIDE POLYMORPHISMS (SNPS) IN THE ACTINIDIA CHINENSIS SPECIES COMPLEX (ACTINIDIACEAE)
	Zhou, Jin 2,3; Liu, Yifei 1; Huang, Hongwen 1
刊名	AMERICAN JOURNAL OF BOTANY
	2011-05-01
卷号	98 期号:5 页码:E100-E102
关键词	Actinidia Cleaved Amplified polymorphic Sequences (CAPS) Expressed Sequence Tags (EST) Single Nucleotide Polymorphism (SNP)
ISSN号	0002-9122
DOI	10.3732/ajb.1000442
英文摘要	. Premise of the study : Based on the ESTs database, we report here for the first time 15 polymorphic single nucleotide polymorphisms (SNPs) in the Actinidia chinensis species complex that can be used to study population genetic variation. . Methods and Results : SNPs derived from ESTs of the A. chinensis species complex were identified by the in silico SNP discovery strategy. The Cleaved Amplified polymorphic Sequences (CAPS) method was used to validate putative SNPs in a sample of 24 diploid A. chinensis individuals. For the 15 nuclear SNPs identified, all loci had two alleles, and the minor allele frequency ranged from 0.146 to 0.479. The expected and observed heterozygosities ranged from 0 to 0.500 and from 0 to 0.875, respectively. . Conclusions : The developed markers in this study will provide valuable tools for characterizing genetic variation in evolutionary and ecological studies of natural populations.
资助项目	National Natural Science Foundation of China[30900119] ; National Natural Science Foundation of China[30771479] ; Chinese Academy of Sciences[KSCX2-YW-N-061]
WOS研究方向	Plant Sciences
语种	英语
出版者	BOTANICAL SOC AMER INC
WOS记录号	WOS:000290480600001
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/873]
专题	中国科学院武汉植物园
通讯作者	Huang, Hongwen
作者单位	1.Chinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou 510650, Guangdong, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Jin,Liu, Yifei,Huang, Hongwen. CHARACTERIZATION OF 15 NOVEL SINGLE NUCLEOTIDE POLYMORPHISMS (SNPS) IN THE ACTINIDIA CHINENSIS SPECIES COMPLEX (ACTINIDIACEAE)[J]. AMERICAN JOURNAL OF BOTANY,2011,98(5):E100-E102.
APA	Zhou, Jin,Liu, Yifei,&Huang, Hongwen.(2011).CHARACTERIZATION OF 15 NOVEL SINGLE NUCLEOTIDE POLYMORPHISMS (SNPS) IN THE ACTINIDIA CHINENSIS SPECIES COMPLEX (ACTINIDIACEAE).AMERICAN JOURNAL OF BOTANY,98(5),E100-E102.
MLA	Zhou, Jin,et al."CHARACTERIZATION OF 15 NOVEL SINGLE NUCLEOTIDE POLYMORPHISMS (SNPS) IN THE ACTINIDIA CHINENSIS SPECIES COMPLEX (ACTINIDIACEAE)".AMERICAN JOURNAL OF BOTANY 98.5(2011):E100-E102.