Transcriptome analysis based on next-generation sequencing of non-model plants producing specialized metabolites of biotechnological interest

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	Transcriptome analysis based on next-generation sequencing of non-model plants producing specialized metabolites of biotechnological interest
	Xiao, Mei; Zhang, Ye; Chen, Xue; Lee, Eun-Jeong; Barber, Carla J. S.; Chakrabarty, Romit; Desgagne-Penix, Isabel; Haslam, Tegan M.; Kim, Yeon-Bok; Liu, Enwu
刊名	JOURNAL OF BIOTECHNOLOGY
	2013
英文摘要	Plants produce a vast array of specialized metabolites, many of which are used as pharmaceuticals, flavors, fragrances, and other high-value fine chemicals. However, most of these compounds occur in non-model plants for which genomic sequence information is not yet available. The production of a large amount of nucleotide sequence data using next-generation technologies is now relatively fast and cost-effective, especially when using the latest Roche-454 and Illumina sequencers with enhanced base-calling accuracy. To investigate specialized metabolite biosynthesis in non-model plants we have established a data-mining framework, employing next-generation sequencing and computational algorithms, to construct and analyze the transcriptomes of 75 non-model plants that produce compounds of interest for biotechnological applications. After sequence assembly an extensive annotation approach was applied to assign functional information to over 800,000 putative transcripts. The annotation is based on direct searches against public databases, including RefSeq and InterPro. Gene Ontology (GO), Enzyme Commission (EC) annotations and associated Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway maps are also collected. As a proof-of-concept, the selection of biosynthetic gene candidates associated with six specialized metabolic pathways is described. A web-based BLAST server has been established to allow public access to assembled transcriptome databases for all 75 plant species of the PhytoMetaSyn Project (www.phytometasyn.ca). (c) 2013 The Authors. Published by Elsevier B.V. All rights reserved.
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/596]
专题	中国科学院武汉植物园
推荐引用方式 GB/T 7714	Xiao, Mei,Zhang, Ye,Chen, Xue,et al. Transcriptome analysis based on next-generation sequencing of non-model plants producing specialized metabolites of biotechnological interest[J]. JOURNAL OF BIOTECHNOLOGY,2013.
APA	Xiao, Mei.,Zhang, Ye.,Chen, Xue.,Lee, Eun-Jeong.,Barber, Carla J. S..,...&Sensen, Christoph W..(2013).Transcriptome analysis based on next-generation sequencing of non-model plants producing specialized metabolites of biotechnological interest.JOURNAL OF BIOTECHNOLOGY.
MLA	Xiao, Mei,et al."Transcriptome analysis based on next-generation sequencing of non-model plants producing specialized metabolites of biotechnological interest".JOURNAL OF BIOTECHNOLOGY (2013).