Analysis of phosphoproteome in rice pistil

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	Analysis of phosphoproteome in rice pistil
	Wang, Kun; Zhao, Yong; Li, Ming; Gao, Feng; Yang, Ming-kun; Wang, Xin; Li, Shaoqing; Yang, Pingfang
刊名	PROTEOMICS
	2014
英文摘要	As the female reproductive part of a flower, the pistil consists of the ovary, style, and stigma, and is a critical organ for the process from pollen recognition to fertilization and seed formation. Previous studies on pollen-pistil interaction mainly focused on gene expression changes with comparative transcriptomics or proteomics method. However, studies on protein PTMs are still lacking. Here we report a phosphoproteomic study on mature pistil of rice. Using IMAC enrichment, hydrophilic interaction chromatography fraction and high-accuracy MS instrument (TripleTOF 5600), 2347 of high-confidence (Ascore >= 19, p <= 0.01), phosphorylation sites corresponding to 1588 phosphoproteins were identified. Among them, 1369 phosphorylation sites within 654 phosphoproteins were newly identified; 41 serine phosphorylation motifs, which belong to three groups: proline-directed, basophilic, and acidic motifs were identified after analysis by motif-X. Two hundred and one genes whose phosphopeptides were identified here showed tissue-specific expression in pistil based on information mining of previous microarray data. All MS data have been deposited in the ProteomeXchange with identifier PXD000923 (http://proteomecentral.proteomexchange.org/dataset/PXD000923). This study will help us to understand pistil development and pollination on the posttranslational level.
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/369]
专题	中国科学院武汉植物园
推荐引用方式 GB/T 7714	Wang, Kun,Zhao, Yong,Li, Ming,et al. Analysis of phosphoproteome in rice pistil[J]. PROTEOMICS,2014.
APA	Wang, Kun.,Zhao, Yong.,Li, Ming.,Gao, Feng.,Yang, Ming-kun.,...&Yang, Pingfang.(2014).Analysis of phosphoproteome in rice pistil.PROTEOMICS.
MLA	Wang, Kun,et al."Analysis of phosphoproteome in rice pistil".PROTEOMICS (2014).