Proteomic and Epigenetic Analyses of Lotus (Nelumbo nucifera) Petals Between Red and White cultivars

doi:10.1093/pcp/pcv077

CORC > 武汉植物园 > 中国科学院武汉植物园

	Proteomic and Epigenetic Analyses of Lotus (Nelumbo nucifera) Petals Between Red and White cultivars
	Damaris, Rebecca Njeri 1,2; Wang, Kun 2; Yang, Pingfang 2; Li, Tingting 3; Deng, Jiao 1,2; Fu, Ziyang 1,2; Chen, Sha 4
刊名	PLANT AND CELL PHYSIOLOGY
	2015-08-01
卷号	56 期号:8 页码:1546-1555
关键词	Anthocyanin Comparative proteomics DNA methylation Lotus petals
ISSN号	0032-0781
DOI	10.1093/pcp/pcv077
英文摘要	Lotus is a vital aquatic ornamental plant with different flower colors. To explore the flower coloration mechanism in lotus, the constituents and contents of pigments in two lotus cultivars with red and white flowers were analyzed. Although flavones and flavonols were detected in both cultivars, anthocyanins could only be detected in the red cultivar. A comparative proteomics analysis on the flower petals between these two cultivars was conducted. A total of 88 differentially expressed proteins were identified with 36 more abundant and 52 less abundant in the red than in the white cultivar. Among them, four enzymes involved in the anthocyanin pathway were identified, i.e. flavanone 3-hydroxylase, anthocyanidin synthase, anthocyanidin 3-O-glucosyltransferase and glutathione S-transferase. Analysis of the expression patterns of anthocyanin biosynthetic genes indicated that the anthocyanindin synthase (ANS) gene might be the critical gene determining anthocyanin biosynthesis and accumulation in lotus flower. Further analysis showed that different methylation intensities on the promoter sequence of the ANS gene might result in the different flower coloration in the red and white cultivar. This study provides new insights into the mechanism of flower coloration in lotus, and may be helpful in its breeding and germplasm enhancement.
资助项目	Chinese Academy of Science[Y455421Z02]
WOS研究方向	Plant Sciences ; Cell Biology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000359649400008
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/227]
专题	中国科学院武汉植物园
通讯作者	Yang, Pingfang
作者单位	1.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 4.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing 100700, Peoples R China
推荐引用方式 GB/T 7714	Damaris, Rebecca Njeri,Wang, Kun,Yang, Pingfang,et al. Proteomic and Epigenetic Analyses of Lotus (Nelumbo nucifera) Petals Between Red and White cultivars[J]. PLANT AND CELL PHYSIOLOGY,2015,56(8):1546-1555.
APA	Damaris, Rebecca Njeri.,Wang, Kun.,Yang, Pingfang.,Li, Tingting.,Deng, Jiao.,...&Chen, Sha.(2015).Proteomic and Epigenetic Analyses of Lotus (Nelumbo nucifera) Petals Between Red and White cultivars.PLANT AND CELL PHYSIOLOGY,56(8),1546-1555.
MLA	Damaris, Rebecca Njeri,et al."Proteomic and Epigenetic Analyses of Lotus (Nelumbo nucifera) Petals Between Red and White cultivars".PLANT AND CELL PHYSIOLOGY 56.8(2015):1546-1555.