Development of microsatellite markers in Hagenia abyssinica (Bruce) JF Gmel, an endangered tropical tree of eastern Africa, using next-generation sequencing

doi:10.4238/gmr.15028168

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	Development of microsatellite markers in Hagenia abyssinica (Bruce) JF Gmel, an endangered tropical tree of eastern Africa, using next-generation sequencing
	Gichira, A. W.1,2; Long, Z. C.1; Hu, G. W.1; Gituru, R. W.3; Wang, Q. F.1; Chen, J. M.1
刊名	GENETICS AND MOLECULAR RESEARCH
	2016
卷号	15 期号:2 页码:4
关键词	Afromontane Hagenia abyssinica Microsatellites Rosaceae SSR Next-generation sequencing
ISSN号	1676-5680
DOI	10.4238/gmr.15028168
英文摘要	Hagenia abyssinica (Bruce) J. F. Gmel is an endangered tree species endemic to the high mountains of tropical Africa. We used Illumina paired-end technology to sequence its nuclear genome, aiming at creating the first genomic data library and developing the first set of genomic microsatellites. Seventeen microsatellite markers were validated in 24 individuals. The average number of alleles per locus was 7.6, while the observed and expected heterozygosities ranged from 0.000 to 0.958 and from 0.354 to 0.883, respectively. These polymorphic markers will be used as tools for further molecular studies to facilitate formulation of appropriate conservation strategies for this species.
资助项目	Sino-Africa Joint Research Center[Y323771W07] ; Sino-Africa Joint Research Center[SAJC201322]
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
语种	英语
出版者	FUNPEC-EDITORA
WOS记录号	WOS:000384881000050
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/142]
专题	中国科学院武汉植物园
通讯作者	Wang, Q. F.; Chen, J. M.
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Jomo Kenyatta Univ Agr & Technol, Dept Bot, Nairobi, Kenya
推荐引用方式 GB/T 7714	Gichira, A. W.,Long, Z. C.,Hu, G. W.,et al. Development of microsatellite markers in Hagenia abyssinica (Bruce) JF Gmel, an endangered tropical tree of eastern Africa, using next-generation sequencing[J]. GENETICS AND MOLECULAR RESEARCH,2016,15(2):4.
APA	Gichira, A. W.,Long, Z. C.,Hu, G. W.,Gituru, R. W.,Wang, Q. F.,&Chen, J. M..(2016).Development of microsatellite markers in Hagenia abyssinica (Bruce) JF Gmel, an endangered tropical tree of eastern Africa, using next-generation sequencing.GENETICS AND MOLECULAR RESEARCH,15(2),4.
MLA	Gichira, A. W.,et al."Development of microsatellite markers in Hagenia abyssinica (Bruce) JF Gmel, an endangered tropical tree of eastern Africa, using next-generation sequencing".GENETICS AND MOLECULAR RESEARCH 15.2(2016):4.