Establishment and field applications of real-time PCR methods for the quantification of potential MIB-producing cyanobacteria in aquatic systems

doi:10.1007/s10811-015-0529-1

CORC > 武汉植物园 > 中国科学院武汉植物园

	Establishment and field applications of real-time PCR methods for the quantification of potential MIB-producing cyanobacteria in aquatic systems
	Wang, Zhongjie 1,2; Song, Gaofei 2; Shao, Jihai 3; Tan, Wenhua 4; Li, Yeguang 1; Li, Renhui 2
刊名	JOURNAL OF APPLIED PHYCOLOGY
	2016-02-01
卷号	28 期号:1 页码:325-333
关键词	2-Methylisoborneol Taste and odor MIB cyclase gene Real-time PCR Cyanobacteria
ISSN号	0921-8971
DOI	10.1007/s10811-015-0529-1
英文摘要	2-Methylisoborneol (MIB), along with geosmin, has been frequently associated with taste and odor (T&O) events in waters, and cyanobacterial species were considered the main producers in freshwater ecosystems. The detection and quantification of genes responsible for the synthesis of these odors on-site could be valuable for the prediction and treatment of T&O. In this study, SYBR Green and TaqMan real-time quantitative polymerase chain reaction (qPCR) assays were established on the basis of conserved regions in cyanobacterial MIB cyclase gene (mic) and applied to the quantification of potential MIB-producing cyanobacteria in waters. These two qPCR assays showed the prescribed values of amplification efficiency (0.976 and 1.023, respectively) with linearity (R (2) > 0.99) in the range of 10(1) to 10(7) mic copies. Examination on environmental samples and cyanobacterial cultures indicated that qPCRs were rarely inhibited by background biomass, and the limit of quantification was at the level of 10(3) mic copies L-1 in natural waters. The seasonal variations of mic abundances in Lushui Reservoir, Donghu Lake, and Qinghai Lake of China were successfully quantified using the two qPCR protocols and indicated the applicability of these assays in field monitoring. Regression analysis revealed the positive correlation between mic abundance and MIB concentration, but no significant linear correlation was found between MIB-producing cyanobacteria and total cyanobacteria in investigated waters. The qPCR assays developed in this study could rapidly and reliably detect and quantify the abundance of potential cyanobacterial MIB producers, thereby providing a valuable tool for the prediction and early detection of T&O events.
资助项目	NSFC-JST[41261140337] ; National Water Science and Technology Projects[2012ZX07105-004]
WOS研究方向	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000369243300033
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/125]
专题	中国科学院武汉植物园
通讯作者	Li, Renhui
作者单位	1.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan Bot Garden, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China 3.Hunan Agr Univ, Resources & Environm Coll, Changsha 410128, Hunan, Peoples R China 4.Max Planck Inst Chem Ecol, Dept Bioorgan Chem, Hans Knoll Str 8, D-07745 Jena, Germany
推荐引用方式 GB/T 7714	Wang, Zhongjie,Song, Gaofei,Shao, Jihai,et al. Establishment and field applications of real-time PCR methods for the quantification of potential MIB-producing cyanobacteria in aquatic systems[J]. JOURNAL OF APPLIED PHYCOLOGY,2016,28(1):325-333.
APA	Wang, Zhongjie,Song, Gaofei,Shao, Jihai,Tan, Wenhua,Li, Yeguang,&Li, Renhui.(2016).Establishment and field applications of real-time PCR methods for the quantification of potential MIB-producing cyanobacteria in aquatic systems.JOURNAL OF APPLIED PHYCOLOGY,28(1),325-333.
MLA	Wang, Zhongjie,et al."Establishment and field applications of real-time PCR methods for the quantification of potential MIB-producing cyanobacteria in aquatic systems".JOURNAL OF APPLIED PHYCOLOGY 28.1(2016):325-333.