Mining and characterizing EST-SSR markers from ESTs of Torreya grandis

doi:10.1016/j.bse.2016.02.017

CORC > 武汉植物园 > 中国科学院武汉植物园

	Mining and characterizing EST-SSR markers from ESTs of Torreya grandis
	Yi, Guanmei 1; Zhao, Tingting 2; Yin, Zhinang 2; Tang, Ping 2; Qiu, Yingjun 1; Yao, Xiaohong 2
刊名	BIOCHEMICAL SYSTEMATICS AND ECOLOGY
	2016-04-01
卷号	65 页码:124-128
关键词	Torreya grandis Taxaceae EST Microsatellite Endangered species
ISSN号	0305-1978
DOI	10.1016/j.bse.2016.02.017
英文摘要	Torreya grandis Fort. ex Lindl. cv. Merrillii (Taxaceae) is an important economic tree species grown for dried nuts in China. However, it was listed as national second-grade key protected wild plants due to overexploitation of wild resources. To enhance knowledge about genetic characteristics useful for the conservation and sustainable utilization of genetic resource of this species, microsatellite loci were developed by analyzing a reference transcriptome de novo assembled from RNA-seq data of T. grandis. A total of 1713 SSRs containing repeats from di-to pentanucleotides were detected in 46,000 unigenes. Trinucleotide (46.9%) was the most common repeat unit followed by the di (19.7%), pentanucleotides (15.1%), hexa (8.9%), and tetra (6.5%). From these, 108 primer pairs were designed, among which nineteen polymorphic microsatellites were developed and characterized. The number of alleles per locus ranged from 2 to 4, with an average of 2.6 alleles per locus. The observed and expected heterozygosity ranged from 0.10 to 1.00 and 0.13 to 0.70, respectively. Six loci showed significant departure from HWE after Bonferroni correction. The microsatellite markers described here will be useful for evaluating the genetic structure of T. grandis as well as to estimate fine-scale gene flow rates. (C) 2016 Elsevier Ltd. All rights reserved.
资助项目	Natural Scientific Foundation of Ningbo City Government[2015A610267] ; Agricultural Project from Bureau of Science and Technology of Ningbo[2014C11006]
WOS研究方向	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000374599200017
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/87]
专题	中国科学院武汉植物园
通讯作者	Yao, Xiaohong
作者单位	1.Ningbo City Coll Vocat Technol, Ningbo 315000, Zhejiang, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan 430074, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Yi, Guanmei,Zhao, Tingting,Yin, Zhinang,et al. Mining and characterizing EST-SSR markers from ESTs of Torreya grandis[J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2016,65:124-128.
APA	Yi, Guanmei,Zhao, Tingting,Yin, Zhinang,Tang, Ping,Qiu, Yingjun,&Yao, Xiaohong.(2016).Mining and characterizing EST-SSR markers from ESTs of Torreya grandis.BIOCHEMICAL SYSTEMATICS AND ECOLOGY,65,124-128.
MLA	Yi, Guanmei,et al."Mining and characterizing EST-SSR markers from ESTs of Torreya grandis".BIOCHEMICAL SYSTEMATICS AND ECOLOGY 65(2016):124-128.