Evolutionary origin of the NCSI gene subfamily encoding norcoclaurine synthase is associated with the biosynthesis of benzylisoquinoline alkaloids in plants

doi:10.1038/srep26323

CORC > 武汉植物园 > 中国科学院武汉植物园

	Evolutionary origin of the NCSI gene subfamily encoding norcoclaurine synthase is associated with the biosynthesis of benzylisoquinoline alkaloids in plants
	Han, Yuepeng 1,4; Deng, Xianbao 4; Owiti, Albert 2,4; Meelaph, Thitirat 3; Ogutu, Collins 2,4; Vimolmangkang, Sornkanok 3,4
刊名	SCIENTIFIC REPORTS
	2016-05-18
卷号	6 页码:10
ISSN号	2045-2322
DOI	10.1038/srep26323
英文摘要	Sacred lotus is rich in biologically active compounds, particularly benzylisoquinoline alkaloids (BIAs). Here, we report on isolation of genes encoding (S)-norcoclaurine synthase (NCS) in sacred lotus, which is a key entry-enzyme in BIA biosynthesis. Seven NCS genes, designated NnNCS1 through NnNCS7, were identified in the sacred lotus genome, and five are located next to each other within a 83 kb region on scaffold 8. The NCS genes are divided into two subfamilies, designated NCSI and NCSII. The NCSII genes are universal in plants, while the NCSI genes are only identified in a limited number of dicotyledonous taxa that produce BIAs. In sacred lotus, only NnNCS4 belongs to the NCSII subfamily, whilst the rest NCS genes within the NCSI subfamily. Overall, the NnNCS7 gene was predominantly expressed in all tested tissues, and its expression is significantly correlated with alkaloid content in leaf. In contrast, the NnNCS4 expression shows no significant correlation with alkaloid accumulation in leaf, and its lack of expression cannot inhibit alkaloid accumulation. Taken together, these results suggest that the NCSI subfamily is crucial for BIA biosynthesis, and its origin may represent an important evolutionary event that allows certain plant taxa to produce BIAs.
资助项目	National Natural Science Foundation of China[31450110420] ; Chinese Academy of Sciences Visiting Professorship for Young International Scientists[2013FFSB0013] ; Overseas Construction Plan for Science and Education Base, China-Africa Center for Research and Education, Chinese Academy of Sciences[SAJC201327]
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000375998800001
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/72]
专题	中国科学院武汉植物园
通讯作者	Han, Yuepeng
作者单位	1.Chinese Acad Sci, Sinoafrican Joint Res Ctr, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Grad Univ, 19A Yuquanlu, Beijing 100049, Peoples R China 3.Chulalongkorn Univ, Fac Pharmaceut Sci, Dept Pharmacognosy & Pharmaceut Bot, Bangkok 10330, Thailand 4.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Han, Yuepeng,Deng, Xianbao,Owiti, Albert,et al. Evolutionary origin of the NCSI gene subfamily encoding norcoclaurine synthase is associated with the biosynthesis of benzylisoquinoline alkaloids in plants[J]. SCIENTIFIC REPORTS,2016,6:10.
APA	Han, Yuepeng,Deng, Xianbao,Owiti, Albert,Meelaph, Thitirat,Ogutu, Collins,&Vimolmangkang, Sornkanok.(2016).Evolutionary origin of the NCSI gene subfamily encoding norcoclaurine synthase is associated with the biosynthesis of benzylisoquinoline alkaloids in plants.SCIENTIFIC REPORTS,6,10.
MLA	Han, Yuepeng,et al."Evolutionary origin of the NCSI gene subfamily encoding norcoclaurine synthase is associated with the biosynthesis of benzylisoquinoline alkaloids in plants".SCIENTIFIC REPORTS 6(2016):10.