Molecular Cloning and Functional Characterization of a Novel Isoflavone 3 '-O-methyltransferase from Pueraria lobata

doi:10.3389/fpls.2016.00793

CORC > 武汉植物园 > 中国科学院武汉植物园

	Molecular Cloning and Functional Characterization of a Novel Isoflavone 3 '-O-methyltransferase from Pueraria lobata
	Li, Jia 1; Li, Changfu 1; Gou, Junbo 1,2; Zhang, Yansheng 1
刊名	FRONTIERS IN PLANT SCIENCE
	2016-06-06
卷号	7 页码:8
关键词	methyltransferase methyl jasmonate isoflavone 3 '-O-methylation Pueraria lobata
ISSN号	1664-462X
DOI	10.3389/fpls.2016.00793
英文摘要	Pueraria lobate roots accumulate 3'-, 4'- and 7-O-methylated isoflavones and many of these methylated compounds exhibit various pharmacological activities. Either the 4'- or 7-O-methylation activity has been investigated at molecular levels in several legume species. However, the gene encoding the isoflavone 3'-O-methyltransferase (OMT) has not yet been isolated from any plant species. In this study, we reported the first cDNA encoding the isoflavone 3'-OMT from P. lobata (designated PIOMT4). Heterologous expressions in yeast and Escherichia coli cells showed that the gene product exhibits an enzyme activity to methylate the 3'-hydroxy group of the isoflavone substrate. The transcript abundance of PIOMT4 matches well with its enzymatic product in different organs of P lobata and in the plant roots in response to methyl jasmonate elicitation. Integration of the biochemical with metabolic and transcript data supported the proposed function of PIOMT4. The identification of PIOMT4 would not only help to understand the isoflavonoid metabolism in P lobata but also potentially provide an enzyme catalyst for methylating existing drug candidates to improve their hydrophobicity.
资助项目	National Natural Science Foundation of China[31300258] ; National Natural Science Foundation of China[31170284] ; One Hundred Talent Program of the CAS[29Y129441R0104444]
WOS研究方向	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000377071100001
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/63]
专题	中国科学院武汉植物园
通讯作者	Zhang, Yansheng
作者单位	1.Chinese Acad Sci, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan Bot Garden, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Jia,Li, Changfu,Gou, Junbo,et al. Molecular Cloning and Functional Characterization of a Novel Isoflavone 3 '-O-methyltransferase from Pueraria lobata[J]. FRONTIERS IN PLANT SCIENCE,2016,7:8.
APA	Li, Jia,Li, Changfu,Gou, Junbo,&Zhang, Yansheng.(2016).Molecular Cloning and Functional Characterization of a Novel Isoflavone 3 '-O-methyltransferase from Pueraria lobata.FRONTIERS IN PLANT SCIENCE,7,8.
MLA	Li, Jia,et al."Molecular Cloning and Functional Characterization of a Novel Isoflavone 3 '-O-methyltransferase from Pueraria lobata".FRONTIERS IN PLANT SCIENCE 7(2016):8.