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Molecular cloning and functional analysis of a 10-epi-junenol synthase from Inula hupehensis
Gou, Jun-Bo1,2; Li, Zhen-Qiu3; Li, Chang-Fu1; Chen, Fang-Fang1; Lv, Shi-You1; Zhang, Yan-Sheng1
刊名PLANT PHYSIOLOGY AND BIOCHEMISTRY
2016-09-01
卷号106页码:288-294
关键词10-Epi-junenol synthase Inula hupehensis Eudesmanolide
ISSN号0981-9428
DOI10.1016/j.plaphy.2016.05.023
英文摘要Junenol based-eudesmanolides have been detected in many compositae plant species and were reported to exhibit various pharmacological activities. So far, the gene encoding junenol synthase has never been isolated. Here we report the molecular cloning and functional analysis of a 10-epi-junenol synthase from Inula hupehensis (designated IhsTPS1). IhsTPS1 converts the substrate farnesyl diphosphate into multiple sesquiterpenes with the product 10-epi-junenol being predominant. The transcript levels of IhsTPS1 correlate well with the accumulation pattern of 10-epi-junenol in I. hupehensis organs, supporting its biochemical roles in vivo. (C) 2016 Elsevier Masson SAS. All rights reserved.
资助项目National Natural Science Foundation of China[31370339] ; Grant of One Hundred Talent Program of the CAS[29Y129441R0104444]
WOS研究方向Plant Sciences
语种英语
出版者ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
WOS记录号WOS:000382344900033
内容类型期刊论文
源URL[http://202.127.146.157/handle/2RYDP1HH/36]  
专题中国科学院武汉植物园
通讯作者Zhang, Yan-Sheng
作者单位1.Chinese Acad Sci, Wuhan Bot Garden, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan 430071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Hebei Univ, Coll Life Sci, Baoding 071002, Peoples R China
推荐引用方式
GB/T 7714
Gou, Jun-Bo,Li, Zhen-Qiu,Li, Chang-Fu,et al. Molecular cloning and functional analysis of a 10-epi-junenol synthase from Inula hupehensis[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2016,106:288-294.
APA Gou, Jun-Bo,Li, Zhen-Qiu,Li, Chang-Fu,Chen, Fang-Fang,Lv, Shi-You,&Zhang, Yan-Sheng.(2016).Molecular cloning and functional analysis of a 10-epi-junenol synthase from Inula hupehensis.PLANT PHYSIOLOGY AND BIOCHEMISTRY,106,288-294.
MLA Gou, Jun-Bo,et al."Molecular cloning and functional analysis of a 10-epi-junenol synthase from Inula hupehensis".PLANT PHYSIOLOGY AND BIOCHEMISTRY 106(2016):288-294.
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