Compound-Specific Isotope Analysis of Amino Acid Labeling with Stable Isotope Nitrogen (N-15) in Higher Plants
Xiaofei Gao; Zhongyi Zhang; Huayun Xiao; Nengjian Zheng; RenGuo Zhu
刊名Chromatographia
2016
卷号79期号:17-18页码:1197-1205
英文摘要

Individual free amino acid delta N-15 values in plant tissue reflect the metabolic pathways involved in their biosynthesis and catabolism and could thus aid understanding of environmental stress and anthropogenic effects on plant metabolism. In this study, compound-specific nitrogen isotope analysis of amino acid by gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS) was carried out to determine individual free amino acid delta N-15 values. High correlations were observed between the delta N-15 values obtained by GC-C-IRMS and elemental analyzer-isotope ratio mass spectrometry (EA-IRMS) determinations, and the mean precision measured was better than 1 aEuro degrees. Cation-exchange chromatography was employed to purify the sample, and the difference between prior to and following passage through the resin was within 1 aEuro degrees. The amino acid delta N-15 values of plant leave samples following incubation in N-15-nitrate at different time points were determined. A typical foliar free amino acid N-15-enrichment pattern was found, and glutamine was the most rapidly labeled amino acid; other amino acids derived from the GS-GOGAT cycle were also enriched. The pyruvate family amino acids were labeled less quickly followed by the aromatic amino acids. This study highlighted that amino acid metabolism pathways had a major effect on the delta N-15 values. With the known amino acid metabolism pathways and delta N-15 values determined by the presented method, the influence of various external factors on the metabolic cycling of amino acid can be understood well.

学科主题环境地球化学
语种英语
公开日期2017-11-21
内容类型期刊论文
源URL[http://ir.gyig.ac.cn:8080/handle/352002/6534]  
专题地球化学研究所_环境地球化学国家重点实验室_环境地球化学国家重点实验室_期刊论文
推荐引用方式
GB/T 7714
Xiaofei Gao,Zhongyi Zhang,Huayun Xiao,et al. Compound-Specific Isotope Analysis of Amino Acid Labeling with Stable Isotope Nitrogen (N-15) in Higher Plants[J]. Chromatographia,2016,79(17-18):1197-1205.
APA Xiaofei Gao,Zhongyi Zhang,Huayun Xiao,Nengjian Zheng,&RenGuo Zhu.(2016).Compound-Specific Isotope Analysis of Amino Acid Labeling with Stable Isotope Nitrogen (N-15) in Higher Plants.Chromatographia,79(17-18),1197-1205.
MLA Xiaofei Gao,et al."Compound-Specific Isotope Analysis of Amino Acid Labeling with Stable Isotope Nitrogen (N-15) in Higher Plants".Chromatographia 79.17-18(2016):1197-1205.
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