Synthesis of magnetic zwitterionic–hydrophilic material for the selective enrichment of N-linked glycopeptides

doi:10.1016/j.chroma.2016.12.054

	Synthesis of magnetic zwitterionic–hydrophilic material for the selective enrichment of N-linked glycopeptides
	Zhao, Yiman 1; Chen, Yajing 1; Xiong, Zhichao 2; Sun, Xudong 1; Zhang, Quanqing 3; Gan, Yangyang 1; Zhang, Lingyi 1; Zhang, Weibing 1
刊名	Journal of Chromatography A
	2017
卷号	1482 页码:23-31
ISSN号	00219673
DOI	10.1016/j.chroma.2016.12.054
英文摘要	In consideration of the close connection between glycopeptides and human diseases, the efficient method to separate and enrich glycopeptides from complex biological samples is urgently required. In the work, we developed a magnetic zwitterionic-hydrophilic material for highly effective separation and analysis of glycopeptides from complex samples. The Fe3O4particles were covered with a thick layer of polymer by one-step reflux-precipitation polymerization (RPP), subsequently decorated by Au nanoparticles (Au NPs) through in situ reduction and finally modified with zwitterionic groups. The abundant zwitterionic sites facilitate the selective enrichment of glycopeptides. Besides, the prepared Fe3O4@PGMA@Au-L-cys showed high detection sensitivity (5 fmol IgG digest), approving enrichment capacity (75 mg g−1), satisfactory enrichment recovery (89.8%), and great performance in the analysis and profiling of low-abundance N-linked glycopeptides. Furthermore, the prepared material was employed in the enrichment of glycopeptides in intricate biological samples, and 774 unique N-glycosylation sites from 411 N-glycosylated proteins were reliably identified in three replicate analyses of a 75 μg protein sample extracted from mouse liver, suggesting wide application prospect in glycoproteomics. © 2016 Elsevier B.V.
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/25653]
专题	中国科学院上海硅酸盐研究所
作者单位	1.Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai; 200237, China; 2.State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai; 200050, China; 3.Fudan University, Shanghai; 200433, China
推荐引用方式 GB/T 7714	Zhao, Yiman,Chen, Yajing,Xiong, Zhichao,et al. Synthesis of magnetic zwitterionic–hydrophilic material for the selective enrichment of N-linked glycopeptides[J]. Journal of Chromatography A,2017,1482:23-31.
APA	Zhao, Yiman.,Chen, Yajing.,Xiong, Zhichao.,Sun, Xudong.,Zhang, Quanqing.,...&Zhang, Weibing.(2017).Synthesis of magnetic zwitterionic–hydrophilic material for the selective enrichment of N-linked glycopeptides.Journal of Chromatography A,1482,23-31.
MLA	Zhao, Yiman,et al."Synthesis of magnetic zwitterionic–hydrophilic material for the selective enrichment of N-linked glycopeptides".Journal of Chromatography A 1482(2017):23-31.