Electrospinning of PVA/sericin nanofiber and the effect on epithelial-mesenchymal transition of A549 cells

doi:10.1016/j.msec.2017.05.048

	Electrospinning of PVA/sericin nanofiber and the effect on epithelial-mesenchymal transition of A549 cells
	Yan, Shanshan 1,3,4; Li, Xiuchun 1; Dai, Jing 1; Wang, Yiqun 1; Wang, Binbin 2,4,5; Lu, Yi 1; Shi, Jianlin 1,3; Huang, Pengyu 2; Gong, Jinkang 1; Yao, Yuan 1
刊名	Materials Science and Engineering C
	2017
卷号	79 页码:436-444
ISSN号	09284931
DOI	10.1016/j.msec.2017.05.048
英文摘要	This research aims to investigate the cell-nanomaterial interaction between epithelial-mesenchymal transition of A549 cell and electrospinning nanofibers composed of polyvinyl alcohol (PVA)/silk sericin (SS). The electrospinning of regenerated nanofiber was performed with water as a spinning solvent and glutaraldehyde as a chemical cross-linker. Solution concentration, applied voltage and spin distances as well as other parameters were optimized to generate fine nanofibers with smooth surface in good homogeneity. From the scanning electron microscopy (SEM) analysis, the nanofibers had an average diameter of 200 nm. Epithelial-mesenchymal transition (EMT) is a process by which epithelial cells lose their cell polarity to become mesenchymal stem cells. This transition is affected by multiple biochemical and physical factors in cell metabolism cascade. Herein, we investigate the biophysical effect on A549 EMT by culturing cells on nanofibrous mats with different topography and composition. The cell viability was evaluated by biochemical assay and its morphology was observed with SEM. The results demonstrate that cells appropriately attached to the surface of the nanofibrous mats with extended morphology by their filopodia. Gene expression analysis was conducted by real-time PCR using multiple markers for detecting EMT: N-cadherin (NCad), Vimentin (Vim), Fibronectin (Fib) and Matrix metallopeptidase (MMP9). An increasing expression pattern was observed on NCad, Vim, Fib, with respect to a negative control as cell cultured on polystyrene dish. This result indicates the 200 nm PVA/SS nanofibers may induce A549 cells to process epithelial–mesenchymal transition during the culturing. © 2017 Elsevier B.V.
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/25665]
专题	中国科学院上海硅酸盐研究所
作者单位	1.School of Physical Science and Technology, ShanghaiTech University, 393 Middle Huaxia Road, Pudong, Shanghai; 201210, China; 2.School of Life Science and Technology, ShanghaiTech University, 393 Middle Huaxia Road, Pudong, Shanghai; 201210, China; 3.Shanghai Institute of Ceramics, Chinese Academy of Science, 1295 Dingxi Road, Changning, Shanghai; 200050, China; 4.University of Chinese Academy of Sciences, 19 Yuquan Road, Shijingshan, Beijing; 100049, China; 5.Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Xuhui, Shanghai; 200031, China
推荐引用方式 GB/T 7714	Yan, Shanshan,Li, Xiuchun,Dai, Jing,et al. Electrospinning of PVA/sericin nanofiber and the effect on epithelial-mesenchymal transition of A549 cells[J]. Materials Science and Engineering C,2017,79:436-444.
APA	Yan, Shanshan.,Li, Xiuchun.,Dai, Jing.,Wang, Yiqun.,Wang, Binbin.,...&Yao, Yuan.(2017).Electrospinning of PVA/sericin nanofiber and the effect on epithelial-mesenchymal transition of A549 cells.Materials Science and Engineering C,79,436-444.
MLA	Yan, Shanshan,et al."Electrospinning of PVA/sericin nanofiber and the effect on epithelial-mesenchymal transition of A549 cells".Materials Science and Engineering C 79(2017):436-444.