YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure

doi:10.1016/j.colsurfb.2017.01.039

	YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure
	Pan, Houhua 1,2,3; Xie, Youtao 1; Zhang, Zequan 1; Li, Kai 1; Hu, Dandan 1; Zheng, Xuebin 1; Fan, Qiming 2; Tang, Tingting 2
刊名	Colloids and Surfaces B: Biointerfaces
	2017
卷号	152 页码:344-353
ISSN号	09277765
DOI	10.1016/j.colsurfb.2017.01.039
英文摘要	Hierarchical structure mimicking the natural bone microenvironment has been considered as a promising platform to regulate cell functions. We have previously fabricated hierarchical macropore/nanowire structure and evidence has shown that it can better manipulate the cytoskeleton status and osteogenic performance of osteoblasts. However, how cues of hierarchical structure are translated and ultimately linked to BMSC lineage commitment have still remained elusive, which hinders the accurate knowledge and further development of the hierarchical structure. In this study, bone marrow-derived mesenchymal stem cells (BMSCs) fate on hierarchical structure was investigated as well as the detailed mechanisms. It was shown that well-developed cytoskeleton and focal adhesion were observed for BMSCs on hierarchical structure, which was accompanied by enhanced osteogenic and depressed adipogenic potential. Evidence of increased YAP activity and nuclear translocation were exhibited on hierarchical structure and YAP knockdown inhibited osteogenic differentiation and promoted adipogenic differentiation induced by hierarchical structure. Further remove of cytoskeleton tension inhibited YAP function, which confirmed the key role of YAP-mediated mechanotransduction in the BMSC differentiation. These results together provide information of the stem cell fate commitment on hierarchical structure and a promising approach to design advanced biomaterials by focusing on specific mechanotransduction process. © 2017 Elsevier B.V.
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/25728]
专题	中国科学院上海硅酸盐研究所
作者单位	1.Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai; 200050, China; 2.Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai; 200011, China; 3.University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing; 100049, China
推荐引用方式 GB/T 7714	Pan, Houhua,Xie, Youtao,Zhang, Zequan,et al. YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure[J]. Colloids and Surfaces B: Biointerfaces,2017,152:344-353.
APA	Pan, Houhua.,Xie, Youtao.,Zhang, Zequan.,Li, Kai.,Hu, Dandan.,...&Tang, Tingting.(2017).YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure.Colloids and Surfaces B: Biointerfaces,152,344-353.
MLA	Pan, Houhua,et al."YAP-mediated mechanotransduction regulates osteogenic and adipogenic differentiation of BMSCs on hierarchical structure".Colloids and Surfaces B: Biointerfaces 152(2017):344-353.