Integrated massively parallel sequencing of 15 autosomal STRs and Amelogenin using a simplified library preparation approach

doi:10.1002/elps.201700429

	Integrated massively parallel sequencing of 15 autosomal STRs and Amelogenin using a simplified library preparation approach
	Xue, J; Wu, RG; Pan, YJ; Wang, SX; Qu, BW; Qin, Y; Shi, YQ; Zhang, CC; Li, R; Zhang, LY
刊名	ELECTROPHORESIS
	2018
卷号	39 期号:12 页码:1466
关键词	Forensic genetics GENOME MACHINE PGM Fusion primer FORENSIC APPLICATION Next-generation sequencing HUMAN IDENTIFICATION Short tandem repeat (STR) typing MISEQ POLYMORPHISM SYSTEM
ISSN号	0173-0835
DOI	10.1002/elps.201700429
文献子类	Article
英文摘要	Massively parallel sequencing (MPS) technologies, also termed as next-generation sequencing (NGS), are becoming increasingly popular in study of short tandem repeats (STR). However, current library preparation methods are usually based on ligation or two-round PCR that requires more steps, making it time-consuming (about 2 days), laborious and expensive. In this study, a 16-plex STR typing system was designed with fusion primer strategy based on the Ion Torrent S5 XL platform which could effectively resolve the above challenges for forensic DNA database-type samples (bloodstains, saliva stains, etc.). The efficiency of this system was tested in 253 Han Chinese participants. The libraries were prepared without DNA isolation and adapter ligation, and the whole process only required approximately 5 h. The proportion of thoroughly genotyped samples in which all the 16 loci were successfully genotyped was 86% (220/256). Of the samples, 99.7% showed 100% concordance between NGS-based STR typing and capillary electrophoresis (CE)-based STR typing. The inconsistency might have been caused by off-ladder alleles and mutations in primer binding sites. Overall, this panel enabled the large-scale genotyping of the DNA samples with controlled quality and quantity because it is asimple, operation-friendly process flow that saves labor,time and costs.
WOS记录号	WOS:000435778800006
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/26722]
专题	中国科学院过程工程研究所
推荐引用方式 GB/T 7714	Xue, J,Wu, RG,Pan, YJ,et al. Integrated massively parallel sequencing of 15 autosomal STRs and Amelogenin using a simplified library preparation approach[J]. ELECTROPHORESIS,2018,39(12):1466, 1473.
APA	Xue, J.,Wu, RG.,Pan, YJ.,Wang, SX.,Qu, BW.,...&Sun, Hongyu.(2018).Integrated massively parallel sequencing of 15 autosomal STRs and Amelogenin using a simplified library preparation approach.ELECTROPHORESIS,39(12),1466.
MLA	Xue, J,et al."Integrated massively parallel sequencing of 15 autosomal STRs and Amelogenin using a simplified library preparation approach".ELECTROPHORESIS 39.12(2018):1466.