Conjugation of chitosan oligosaccharides enhances immune response to porcine circovirus vaccine by activating macrophages | |
Zhang, Guiqiang1,2,3; Jia, Peiyuan2,3; Liu, Hongtao2,3; Hu, Tao2,3; Du, Yuguang2,3 | |
刊名 | IMMUNOBIOLOGY |
2018-11-01 | |
卷号 | 223期号:11页码:663-670 |
关键词 | Adjuvant Chitosan Oligosaccharide Conjugation Porcine Circovirus Type 2 (Pcv2) Macrophages Toll-like Receptors |
ISSN号 | 0171-2985 |
DOI | 10.1016/j.imbio.2018.07.012 |
英文摘要 | Porcine circovirus type 2 (PCV2)-associated diseases have led to great economic losses to the pig industry. Our lab previously found that conjugation of chitosan oligosaccharides (COS) or via a carrier protein enhanced the immunogenicity of PCV2 vaccine against infectious pathogens. However, precise mechanisms and signal transduction pathways underlying the efficacy of COS conjugation remains poorly defined. In this study, to better understand the effects and mechanism of COS conjugates maintain the adjuvant potential in vivo, we investigated its augmentation of macrophage function, including cell activation, NO production, cytokine production and phagocytosis. Additionally, the role of Toll-like receptors (TLR) proteins in this process was also assessed. The results indicate that, as compared to the PCV and PCV/COS, conjugation of COS effectively enhanced the NO production, cytokines generation and phagocytosis activity of macrophages. Noticeably, the generation of NO and proinflammatory cytokines was closely related to the TLR2/4 signaling pathways, strongly suggesting that conjugation of COS regulates innate and adaptive immunity by activation of macrophages, resulting in immune enhancement. In summary, the present study provides a potential mechanism of COS conjugation as a novel adjuvant to improve immune responses against various diseases. |
资助项目 | National Key Research and Development Program of China[2017YFD0502303] ; National Natural Science Fund, China[31500747] ; National Natural Science Fund, China[31570801] ; National Natural Science Fund, China[U160820020] |
WOS关键词 | Dendritic Cells ; Porphyra-haitanensis ; Cross-presentation ; Type-2 Pcv2 ; Receptor 4 ; In-vivo ; Innate ; Oligochitosan ; Inflammasome ; Mechanisms |
WOS研究方向 | Immunology |
语种 | 英语 |
出版者 | ELSEVIER GMBH, URBAN & FISCHER VERLAG |
WOS记录号 | WOS:000446807400008 |
资助机构 | National Key Research and Development Program of China ; National Natural Science Fund, China |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/26322] |
专题 | 中国科学院过程工程研究所 |
通讯作者 | Liu, Hongtao; Hu, Tao; Du, Yuguang |
作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Key Lab Biopharmaceut Prod & Formulat Engn, PLA, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Guiqiang,Jia, Peiyuan,Liu, Hongtao,et al. Conjugation of chitosan oligosaccharides enhances immune response to porcine circovirus vaccine by activating macrophages[J]. IMMUNOBIOLOGY,2018,223(11):663-670. |
APA | Zhang, Guiqiang,Jia, Peiyuan,Liu, Hongtao,Hu, Tao,&Du, Yuguang.(2018).Conjugation of chitosan oligosaccharides enhances immune response to porcine circovirus vaccine by activating macrophages.IMMUNOBIOLOGY,223(11),663-670. |
MLA | Zhang, Guiqiang,et al."Conjugation of chitosan oligosaccharides enhances immune response to porcine circovirus vaccine by activating macrophages".IMMUNOBIOLOGY 223.11(2018):663-670. |
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