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	Fe-deficiency-induced expression of bHLH104 enhances Fe-deficiency tolerance of Arabidopsis thaliana
	Wang, Ce; Yao, Xiani; Yu, Diqiu; Liang, Gang
刊名	PLANTA
	2017
卷号	246期号:3页码:421-431
英文摘要	Main conclusion Expression of bHLH104 - GFP driven by the MYB72 promoter improves plants' tolerance to Fe deficiency and increases seed Fe concentrations. Iron (Fe) deficiency causes reduced crop yield and quality. In humans, Fe deficiency is directly associated with Fe-deficiency anemia. Therefore, breeding Fe-deficiency tolerant and Fe-enriched plants are an ideal approach to deal with these problems. Here, different strategies were explored to generate Fe-deficiency tolerant and Fe-enriched plants. Unexpectedly, the overexpression of Fe-deficiency responsive genes (IRT1, MYB72, and bHLH100) resulted in enhanced sensitivity to Fe deficiency, including leaf chlorosis and short roots under Fe-deficiency conditions. Next, three different types of Fe-deficiency responsive promoters (Pro(IRT1) , Pro(MYB72,) and Pro(bHLH100) ) were used to drive the expression of bHLH104-GFP fusion gene in Arabidopsis. Pro(IRT1) :bHLH104-GFP plants showed the enhanced sensitivity to Fe deficiency on Fe-deficient media and the reduced fertility in alkaline soil. In contrast, Pro(bHLH100) :bHLH104-GFP plants displayed a slight tolerance to Fe deficiency and Pro (MYB72) :bHLH104-GFP plants had a significant advantage in growth in alkaline soil, including increased root length, chlorophyll, and biomass. Further analysis revealed that the expression of Fe-deficiency responsive genes was dramatically upregulated in both Pro(MYB72) :bHLH104-GFP and Pro(bHLH100) :bHLH104-GFP plants under Fe-deficiency conditions. When grown in alkaline soil, Pro(MYB72) :bHLH104-GFP plants greatly improved the seed yield and Fe concentration. These results are fundamental for plant manipulation approaches to modify tolerance to Fe deficiency and Fe accumulation through alterations of bHLH104 gene expression.
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语种	英语
公开日期	2017-09-29
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/10595]
专题	西双版纳热带植物园_植物分子生物学研究组 西双版纳热带植物园_2012年后新成立研究组
推荐引用方式 GB/T 7714	Wang, Ce,Yao, Xiani,Yu, Diqiu,et al. Fe-deficiency-induced expression of bHLH104 enhances Fe-deficiency tolerance of Arabidopsis thaliana[J]. PLANTA,2017,246(3):421-431.
APA	Wang, Ce,Yao, Xiani,Yu, Diqiu,&Liang, Gang.(2017).Fe-deficiency-induced expression of bHLH104 enhances Fe-deficiency tolerance of Arabidopsis thaliana.PLANTA,246(3),421-431.
MLA	Wang, Ce,et al."Fe-deficiency-induced expression of bHLH104 enhances Fe-deficiency tolerance of Arabidopsis thaliana".PLANTA 246.3(2017):421-431.

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