Arabidopsis WRKY2 and WRKY34 transcription factors interact with VQ20 protein to modulate pollen development and function

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 植物分子生物学研究组

	Arabidopsis WRKY2 and WRKY34 transcription factors interact with VQ20 protein to modulate pollen development and function
	Lei, Rihua; Li, Xiaoli; Ma, Zhenbing; Lv, Yan; Hu, Yanru; Yu, Diqiu
刊名	PLANT JOURNAL
	2017
卷号	91 期号:6 页码:962-976
英文摘要	Plant male gametogenesis is tightly regulated, and involves complex and precise regulations of transcriptional reprogramming. WRKY transcription factors have been demonstrated to play critical roles in plant development and stress responses. Several members of this family physically interact with VQ motif-containing proteins (VQ proteins) to mediate a plethora of programs in Arabidopsis; however, the involvement of WRKY-VQ complexes in plant male gametogenesis remains largely unknown. In this study, we found that WRKY2 and WKRY34 interact with VQ20 both in vitro and in vivo. Further experiments displayed that the conserved VQ motif of VQ20 is responsible for their physical interactions. The VQ20 protein localizes in the nucleus and specifically expresses in pollens. Phenotypic analysis showed that WRKY2, WRKY34 and VQ20 are crucial for pollen development and function. Mutations of WRKY2, WRKY34 and VQ20 simultaneously resulted in male sterility, with defects in pollen development, germination and tube growth. Further investigation revealed that VQ20 affects the transcriptional functions of its interacting WRKY partners. Complementation evidence supported that the VQ motif of VQ20 is essential for pollen development, as a mutant form of VQ20 in which LVQK residues in the VQ motif were replaced by EDLE did not rescue the phenotype of the w2-1 w34-1 vq20-1 triple-mutant plants. Further expression analysis indicated that WRKY2, WRKY34 and VQ20 co-modulate multiple genes involved in pollen development, germination and tube growth. Taken together, our study provides evidence that VQ20 acts as a key partner of WRKY2 and WKRY34 in plant male gametogenesis.
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语种	英语
公开日期	2017-09-29
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/10591]
专题	西双版纳热带植物园_植物分子生物学研究组西双版纳热带植物园_2012年后新成立研究组
推荐引用方式 GB/T 7714	Lei, Rihua,Li, Xiaoli,Ma, Zhenbing,et al. Arabidopsis WRKY2 and WRKY34 transcription factors interact with VQ20 protein to modulate pollen development and function[J]. PLANT JOURNAL,2017,91(6):962-976.
APA	Lei, Rihua,Li, Xiaoli,Ma, Zhenbing,Lv, Yan,Hu, Yanru,&Yu, Diqiu.(2017).Arabidopsis WRKY2 and WRKY34 transcription factors interact with VQ20 protein to modulate pollen development and function.PLANT JOURNAL,91(6),962-976.
MLA	Lei, Rihua,et al."Arabidopsis WRKY2 and WRKY34 transcription factors interact with VQ20 protein to modulate pollen development and function".PLANT JOURNAL 91.6(2017):962-976.