Programming Cells for Dynamic Assembly of Inorganic Nano-Objects with Spatiotemporal Control

doi:10.1002/adma.201705968

	Programming Cells for Dynamic Assembly of Inorganic Nano-Objects with Spatiotemporal Control
	Wang, Xinyu 1,2; Pu, Jiahua 2,3; An, Bolin 2,4; Li, Yingfeng 1,2; Shang, Yuequn 1,2; Ning, Zhijun ; Liu, Yi 5; Ba, Fang 5; Zhang, Jiaming ; Zhong, Chao
刊名	ADVANCED MATERIALS
	2018
卷号	30 期号:16
关键词	bacterial biofilms curli nanofibers dynamic self-assembly functional amyloids light-induced gene circuits
ISSN号	0935-9648
DOI	10.1002/adma.201705968
英文摘要	Programming living cells to organize inorganic nano-objects (NOs) in a spatiotemporally precise fashion would advance new techniques for creating ordered ensembles of NOs and new bio-abiotic hybrid materials with emerging functionalities. Bacterial cells often grow in cellular communities called biofilms. Here, a strategy is reported for programming dynamic biofilm formation for the synchronized assembly of discrete NOs or hetero-nanostructures on diverse interfaces in a dynamic, scalable, and hierarchical fashion. By engineering Escherichia coli to sense blue light and respond by producing biofilm curli fibers, biofilm formation is spatially controlled and the patterned NOs' assembly is simultaneously achieved. Diverse and complex fluorescent quantum dot patterns with a minimum patterning resolution of 100 mu m are demonstrated. By temporally controlling the sequential addition of NOs into the culture, multilayered heterostructured thin films are fabricated through autonomous layer-by-layer assembly. It is demonstrated that biologically dynamic self-assembly can be used to advance a new repertoire of nanotechnologies and materials with increasing complexity that would be otherwise challenging to produce.
学科主题	Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
出版者	WILEY-V C H VERLAG GMBH
WOS记录号	WOS:000430460100009
资助机构	X.W., J.P., and B.A. contributed equally to this work. The authors thank the Lu group @ MIT for providing Escherichia coli MG1655 PRO Delta csgA and E. coli MG1655 PRO Delta csgA ompR234 cells harboring a pZA-CmR-rr12-pL(tetO)-csgAHis plasmid (TcReceiver/CsgAHis cells). The authors thank Xinliang Mou from JOEL, Beijing for performing HRTEM, HAADF, and EDS mapping. The authors thank Kang Wang (undergraduate, ShanghaiTech University) and Chen Zhang (master student, ShanghaiTech University) for construction of the blue LED setup. Regular TEM characterization was performed at the National Center for Protein Science Shanghai. The Confocal Laser Scanning Microscope was performed at Molecular Imaging Core Facility of SLST, Shanghaitech University. This work was partially sponsored by the Commission for Science and Technology of Shanghai Municipality (Grant No. 17JC1403900), the "Dawn" Program of Shanghai Education Commission, China (Grant No. 14SG56) the Joint Funds of the National Natural Science Foundation of China (Seed Grant No. U1532127), and National Natural Science Foundation of China (Grant No. 31570972) for C.Z. C.Z. acknowledges start-up funding support from ShanghaiTech University and 1000 Youth Talents Program, granted by the Chinese Central Government. C.Z. also thanks Karen Pepper from MIT for revision of the manuscript. ; X.W., J.P., and B.A. contributed equally to this work. The authors thank the Lu group @ MIT for providing Escherichia coli MG1655 PRO Delta csgA and E. coli MG1655 PRO Delta csgA ompR234 cells harboring a pZA-CmR-rr12-pL(tetO)-csgAHis plasmid (TcReceiver/CsgAHis cells). The authors thank Xinliang Mou from JOEL, Beijing for performing HRTEM, HAADF, and EDS mapping. The authors thank Kang Wang (undergraduate, ShanghaiTech University) and Chen Zhang (master student, ShanghaiTech University) for construction of the blue LED setup. Regular TEM characterization was performed at the National Center for Protein Science Shanghai. The Confocal Laser Scanning Microscope was performed at Molecular Imaging Core Facility of SLST, Shanghaitech University. This work was partially sponsored by the Commission for Science and Technology of Shanghai Municipality (Grant No. 17JC1403900), the "Dawn" Program of Shanghai Education Commission, China (Grant No. 14SG56) the Joint Funds of the National Natural Science Foundation of China (Seed Grant No. U1532127), and National Natural Science Foundation of China (Grant No. 31570972) for C.Z. C.Z. acknowledges start-up funding support from ShanghaiTech University and 1000 Youth Talents Program, granted by the Chinese Central Government. C.Z. also thanks Karen Pepper from MIT for revision of the manuscript.
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/24984]
专题	中国科学院上海硅酸盐研究所
作者单位	1.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Ceram, Shanghai 200050, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai 201210, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China 6.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xinyu,Pu, Jiahua,An, Bolin,et al. Programming Cells for Dynamic Assembly of Inorganic Nano-Objects with Spatiotemporal Control[J]. ADVANCED MATERIALS,2018,30(16).
APA	Wang, Xinyu.,Pu, Jiahua.,An, Bolin.,Li, Yingfeng.,Shang, Yuequn.,...&Zhong, Chao.(2018).Programming Cells for Dynamic Assembly of Inorganic Nano-Objects with Spatiotemporal Control.ADVANCED MATERIALS,30(16).
MLA	Wang, Xinyu,et al."Programming Cells for Dynamic Assembly of Inorganic Nano-Objects with Spatiotemporal Control".ADVANCED MATERIALS 30.16(2018).