Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk

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	Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk
	Lai, Weihua; Han, Jiaojiao; Zhang, Lei; Hu, Liming; Xing, Keyu; Lu, Xuefei; Huang, Youju; Zhang, Jiawei; Chen, Tao
刊名	JOURNAL OF DAIRY SCIENCE
	2018
卷号	101 期号:7 页码:5770-5779
关键词	E. Coli O157h7 Listeria-monocytogenes Nanoparticles Amplification Immunoassay Diagnosis Strip Infections Biosensor O157/h7
英文摘要	Lateral flow assay (LFA) has been applied in many fields due to its relative ease of use and cost-effectiveness. However, it has low sensitivity and its applications are limited. Probe materials play a significant role in improving the detection efficiency and sensitivity of LFA. In this study, by using concave palladium-platinum (Pd-Pt) nanoparticles as a nanozyme probe, we developed a sensitive LFA based on the sandwich format for qualitative and quantitative detection of Escherichia coli O157:H7. The sensitivity of the LFA. was improved by applying the 3,3',5,5'-tetramethylbenzidine (TMB) substrate onto the test line where the nanozyme was accumulated in the presence of analytes. The nanozyme showed high catalytic performance toward TMB and greatly enhanced the signal intensity of the test line. The sensitivity of the nanozyme-based LFA was 9.0 x 10(2) cfu/mL in milk, which was 111-fold higher than that of traditional colloidal gold-based LFA. The proposed method has remarkable potential in the detection of various pathogens in real samples.
学科主题	Materials Science ; Physics
语种	英语
公开日期	2018-12-04
内容类型	期刊论文
源URL	[http://ir.nimte.ac.cn/handle/174433/17350]
专题	2018专题
推荐引用方式 GB/T 7714	Lai, Weihua,Han, Jiaojiao,Zhang, Lei,et al. Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk[J]. JOURNAL OF DAIRY SCIENCE,2018,101(7):5770-5779.
APA	Lai, Weihua.,Han, Jiaojiao.,Zhang, Lei.,Hu, Liming.,Xing, Keyu.,...&Chen, Tao.(2018).Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk.JOURNAL OF DAIRY SCIENCE,101(7),5770-5779.
MLA	Lai, Weihua,et al."Nanozyme-based lateral flow assay for the sensitive detection of Escherichia coli O157:H7 in milk".JOURNAL OF DAIRY SCIENCE 101.7(2018):5770-5779.