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题名	不同长径比金纳米锥的光敏剂荧光增强研究及其在光动力疗法上的应用
作者	王诗淼
文献子类	硕士
导师	李儒新
关键词	医用光学 Medical optics 金属增强荧光 Metal-enhanced fluorescence(MEF) 光动力疗法 Photodynamic therapy 金纳米双锥 Gold nanobipyramids (Au NPBs) 等离子体共振 Plasmonic resonance 流式细胞术 Flow cytometry.
其他题名	Aspect Ratio Dependence of the Enhanced Fluorescence Intensity by Gold Nanobipyramids and Its Application on Photodynamic Therapy
英文摘要	金属纳米粒子增强荧光理论和应用研究一直是近年来研究的热点。这种增强荧光光谱被广泛应用于探测、成像、医疗等研究领域。其中一个非常重要的应用就是利用金属纳米粒子结合光敏剂进行光动力治疗的相关研究。在光动力治疗研究过程中，光敏剂作为光动力治疗的三大要素之一 （照射光，光敏剂，氧气） 更是引发了国内外的大量研究。提高光敏剂的效果是其中研究热点之一。金属纳米粒子增强光敏剂荧光的研究对于促进光动力治疗效率具有重要意义。纳米技术的发展促使了纳米粒子在生物医学方面的应用。而金纳米粒子由于稳定的化学性质，良好的光学性质和较低的生物毒性，成为了众多研究者的选择。纳米粒子作为药物载体的研究也发展起来，特别是对于生物体的毒性很低的金纳米粒子，更是受到广泛关注。而金纳米棒由于可以通过调节长径比而比较方便地调节光学性质吸引了越来越多的研究者的兴趣。金纳米锥由于金属的尖端效应而具有更加优异的性质。其两端带有更多正电荷，可以连接更多的光敏剂而增加了其载药功能。 本论文以金纳米锥为研究对象，通过研究不同长径比的金纳米锥，其中金属与酞菁类光敏剂的作用，不仅仅在于表面增强荧光，我们猜测纳米金属材料与酞菁类化合物的共轭物有增强光动力治疗效果的的作用，并根据实验得以验证。本研究采用种子生长法制备，通过在制备过程中通过控制生长液中的硝酸银溶液的含量，制备了长径比分别为3.1，5.1和6.5的金纳米双锥。又利用磺化的铝酞菁作为光敏剂制备了金锥－铝酞菁共轭物，研究了金属-染料纳米复合材料在溶液中的荧光增强作用。实验中我们不仅证明了金锥对于铝酞菁的荧光增强作用，还发现了铝酞菁的荧光发射强度强烈地依赖于金纳米双锥的长径比。结果表明，具有低长径比的金锥会使铝酞菁荧光淬灭，而高长径比的金锥可以获得荧光增强效果（最大增强因子为6）。此外，我们还利用激光扫描共聚焦显微镜进行了细胞中共聚焦荧光增强对比实验，金锥-光敏剂复合体得到了两个数量级的荧光强度增强。基于Hela细胞的凋亡测定的结果证明金锥-铝酞菁共轭物作为药物可以显著增强光动力治疗的效果，并且长径比为6.5的金纳米双锥得到了最好的凋亡结果。本实验的一系列结果为表面增强荧光的研究提供了一个新的途径，并扩展了金属纳米粒子在生命科学领域的应用。; Metal-enhanced fluorescence（MEF）has been a research in recent years. This fluorescence spectroscopy has been widely used in detection, imaging, medical and other fields. Photosensitizer as one of the most important elementsof the three elements (irradiation light, photosensitizer, oxygen) of photodynamic therapy has attracteda large number of domestic and foreign researches. Because improving the effect of photosensitizerwill undoubtedly be more effective for photodynamic therapy. The development of nanotechnology has led to more and more applications of nanoparticles in the field of biomedicine. The gold nanoparticles have become the choice of many researchers because of their stable chemical properties, good optical properties and low biotoxicity. Research of nano-particles as a drug carrier has also developed. Especially the gold nanoparticles due to their low biological toxicity have been widely studied. The gold nanorods have attracted more and more researchers' study because they can adjust the optical properties by changing the aspect ratio. The gold nanobypyramids has more superior properties due to the tip effect of the metal. With more positive charge at both ends, the gold nanobypyramids can be connected to more photosensitizer and then increase its drug-loading function. We conjectured the conjugates of nano-metal materials and phthalocyanine compounds have not only the surface enhancement effect of fluorescence, but also enhancement of thephotodynamic therapy. And it has been verified according to our experiment.Here, the enhancement of dye fluorescence intensity by changing the aspect ratio of gold nanobipyramids (Au NBPs) of 3.1, 5.1 and 6.5 is studied. We find that the emission fluorescence intensities of AlPcS are strongly depending on the aspect ratio of gold nanobipyramids. Au NBPs with low aspect ratio can quench the AlPcS fluorescence. Nonetheless, the MEF in AlPcS- Au NBPs with high aspect ratio can be obtained with an enhancing factor of 6. Furthermore, we also performed confocal fluorescence enhancement contrast in Hela cells, which resulted in twoorders of magnitude enhancement of fluorescence intensity. The result of apoptosis assay based on Hela cells demonstrates the AlPcS-Au NBPs used as a drug can enhance the effect of PDT. And consistently the Au NBPs with the aspect ratio of 6.5 gives the best apoptotic result. These results provide a new avenue for research of surface-enhancement fluorescence and expand the bioapplications of nanoparticles.
学科主题	光学
内容类型	学位论文
源URL	[http://ir.siom.ac.cn/handle/181231/31014]
专题	中国科学院上海光学精密机械研究所
作者单位	中国科学院上海光学精密机械研究所
推荐引用方式 GB/T 7714	王诗淼. 不同长径比金纳米锥的光敏剂荧光增强研究及其在光动力疗法上的应用[D].

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